Shankland G S, Richardson M D
Department of Dermatology, University of Glasgow, UK.
J Antimicrob Chemother. 1990 May;25(5):825-30. doi: 10.1093/jac/25.5.825.
To compare the activity of clotrimazole with a clotrimazole/hydrocortisone combination, guinea pigs were infected with Trichophyton mentagrophytes var. mentagrophytes. The animals were treated with once daily application of clotrimazole alone, hydrocortisone alone, base alone or a combination of clotrimazole and hydrocortisone. One group of animals was left untreated. Daily clinical observations were made. At intervals during the three week study animals were killed, skin scrapings were taken for microscopical examination and culture and skin was removed for histological examination. The responses of the guinea pigs treated with the base alone reflected those of the untreated controls. They were mycologically positive throughout the study and showed extensive inflammatory reactions on histology. Animals treated with hydrocortisone alone rapidly developed a severe clinical infection and mycological examination was positive throughout. Animals treated with clotrimazole alone did not show severe clinical reactions and were negative on culture after four treatments; histology showed inflammatory scabs after resolution of infection. Clinically, the animals receiving combination therapy improved most quickly and cultures were negative after two treatments. Histological examination showed resolution of the infection and little inflammation.
为比较克霉唑与克霉唑/氢化可的松组合的活性，将豚鼠感染须癣毛癣菌须癣变种。动物分别接受以下处理：每日单独应用克霉唑、单独应用氢化可的松、单独应用基质或应用克霉唑与氢化可的松的组合。一组动物不进行治疗。每日进行临床观察。在为期三周的研究期间，每隔一段时间处死动物，取皮肤刮屑进行显微镜检查和培养，并取下皮肤进行组织学检查。单独用基质处理的豚鼠的反应与未处理的对照相似。在整个研究过程中它们的真菌学检查呈阳性，并且在组织学上显示出广泛的炎症反应。单独用氢化可的松处理的动物迅速发展为严重的临床感染，真菌学检查始终呈阳性。单独用克霉唑处理的动物未表现出严重的临床反应，在四次治疗后培养结果为阴性；组织学显示感染消退后有炎性结痂。临床上，接受联合治疗的动物改善最快，两次治疗后培养结果为阴性。组织学检查显示感染消退且炎症轻微。
