Green C J, Sohel I, Vold B S
SRI International, Menlo Park, California 94025.
J Biol Chem. 1990 Jul 25;265(21):12139-42.
Introns in transfer RNA genes are rare in vertebrates. Until now, the only intron-containing human tRNA genes were believed to be those coding for tRNA(Tyr). All of these introns are inserted 3' to the anticodon position in these genes. We have designed polymerase chain reaction primers that can amplify all of the tRNA(Tyr) genes for cloning and sequencing by using the conserved portions of the gene coding for the structural part of the tRNA. Our preliminary results have revealed five tRNA(Tyr) genes, each of which contains a different intron. We used the same technique to amplify, clone, and sequence the human genes for tRNA(Leu)CAA. This has resulted in the discovery that this human tRNA gene family also has introns inserted 3' to the anticodon. This polymerase chain reaction technique is useful in detecting new families of intron-containing tRNA genes as well as identifying sequence variations in the introns of individual genes.
脊椎动物中转运RNA基因的内含子很少见。到目前为止，人们认为唯一含内含子的人类转运RNA基因是那些编码tRNA（Tyr）的基因。所有这些内含子都插入到这些基因反密码子位置的3'端。我们设计了聚合酶链反应引物，通过使用编码tRNA结构部分的基因保守区域，能够扩增所有tRNA（Tyr）基因以进行克隆和测序。我们的初步结果揭示了五个tRNA（Tyr）基因，每个基因都含有一个不同的内含子。我们使用相同的技术扩增、克隆和测序人类tRNA（Leu）CAA基因。这导致发现这个人类转运RNA基因家族也有内含子插入到反密码子的3'端。这种聚合酶链反应技术有助于检测含内含子的转运RNA基因新家族，以及识别单个基因内含子中的序列变异。
