Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Antonie Van Leeuwenhoek. 2013 Jul;104(1):103-10. doi: 10.1007/s10482-013-9930-4. Epub 2013 May 9.
A Gram-negative, aerobic, non-motile and rod-shaped or ovoid bacterial strain, designated D1-W8(T), was isolated from a tidal flat on the South Sea in South Korea. Strain D1-W8(T) was found to grow optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain D1-W8(T) clustered with the type strain of Pelagicola litoralis showing 97.1 % sequence identity. 16S rRNA gene sequences of the type strains of other species exhibited lower similarity values. Strain D1-W8(T) was determined to contain Q-10 as the predominant ubiquinone and C18:1 ω7c as the predominant fatty acid. The major polar lipids of strain D1-W8(T) were identified as phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain D1-W8(T) was determined to be 57.9 mol% and its DNA-DNA relatedness value with the type strain of P. litoralis was 17 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain D1-W8(T) is separate from P. litoralis. On the basis of the data presented, strain D1-W8(T) is considered to represent a novel species of the genus Pelagicola, for which the name Pelagicola litorisediminis sp. nov. is proposed. The type strain is D1-W8(T) (= KCTC 32327(T) = CECT 8287(T)).
一株革兰氏阴性、需氧、不运动、杆状或卵圆形的细菌菌株，被命名为 D1-W8(T)，从韩国南海的潮滩中分离得到。菌株 D1-W8(T) 被发现最适生长温度为 25°C，pH 值为 7.0-8.0，在 2.0-3.0%（w/v）NaCl 存在的条件下生长。基于 16S rRNA 基因序列的邻接法、最大似然法和最大简约法系统发育树表明，菌株 D1-W8(T)与 Pelagicola litoralis 的模式菌株聚在一起，序列同一性为 97.1%。其他种的模式菌株 16S rRNA 基因序列显示出较低的相似性值。D1-W8(T) 菌株被确定含有 Q-10 作为主要的泛醌和 C18:1 ω7c 作为主要的脂肪酸。菌株 D1-W8(T) 的主要极性脂为磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的胺脂和一种未鉴定的脂。D1-W8(T) 菌株的 DNA G+C 含量为 57.9 mol%，与 Pelagicola litoralis 的模式菌株的 DNA-DNA 同源性值为 17%。表型特性的差异，加上系统发育和遗传上的独特性，表明菌株 D1-W8(T)与 Pelagicola litoralis 不同。基于所提供的数据，菌株 D1-W8(T)被认为代表 Pelagicola 属的一个新种，建议将其命名为 Pelagicola litorisediminis sp. nov.。模式菌株为 D1-W8(T)（=KCTC 32327(T)=CECT 8287(T)）。
