Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Antonie Van Leeuwenhoek. 2013 Mar;103(3):465-73. doi: 10.1007/s10482-012-9826-8. Epub 2012 Oct 16.
A Gram-negative, non-motile and rod- or ovoid-shaped bacterial strain, designated YCS-24(T), was isolated from seawater of a seaweed farm in the South Sea, South Korea. Strain YCS-24(T) grew optimally at 25-28 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. Strain YCS-24(T) exhibited the highest 16S rRNA gene sequence similarity values of 97.5 and 97.1 % to the type strains of Thalassobius maritimus and Thalassococcus halodurans, respectively. The neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain YCS-24(T) clustered with the type strain of T. halodurans. The DNA G+C content of strain YCS-24(T) was 58.0 mol% and its DNA-DNA relatedness values with T. halodurans JCM 13833(T) and T. maritimus GSW-M6(T) were 17 ± 6.2 and 23 ± 9.2 %, respectively. The predominant ubiquinone found in strain YCS-24(T) was Q-10 and the predominant fatty acid of strain YCS-24(T) was C(18:1) ω7c. The major polar lipids of strain YCS-24(T) were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain YCS-24(T) is distinguished from T. halodurans. On the basis of the data presented, strain YCS-24(T) (= KCTC 32084(T) = CCUG 62791(T)) represents a novel species of the genus Thalassococcus, for which the name Thalassococcus lentus sp. nov. is proposed.
一株革兰氏阴性、非运动性、杆状或卵圆形的细菌菌株，被命名为 YCS-24(T)，从韩国南海一个海藻养殖场的海水中分离得到。菌株 YCS-24(T) 在 25-28°C、pH7.0-7.5 和 2%（w/v）NaCl 的存在下生长最佳。菌株 YCS-24(T) 与模式菌株 Thalassobius maritimus 和 Thalassococcus halodurans 的 16S rRNA 基因序列相似性最高值分别为 97.5%和 97.1%。基于 16S rRNA 基因序列的邻接法、最大似然法和最大简约法系统发育树显示，菌株 YCS-24(T)与模式菌株 Thalassococcus halodurans 聚为一簇。菌株 YCS-24(T) 的 DNA G+C 含量为 58.0mol%，其与 Thalassococcus halodurans JCM 13833(T) 和 Thalassobius maritimus GSW-M6(T) 的 DNA-DNA 同源性分别为 17±6.2%和 23±9.2%。菌株 YCS-24(T) 中存在的主要醌类为 Q-10，主要脂肪酸为 C(18:1) ω7c。菌株 YCS-24(T) 的主要极性脂类为磷脂酰胆碱、磷脂酰甘油、一种未鉴定的氨基脂类和一种未鉴定的脂类。表型特性的差异，以及系统发育和遗传上的独特性，表明菌株 YCS-24(T)与 Thalassococcus halodurans 不同。基于所提供的数据，菌株 YCS-24(T)=KCTC 32084(T)=CCUG 62791(T)代表了 Thalassococcus 属的一个新种，现提议将其命名为 Lentus 海洋球菌（Lentus thalassococci）。
