Ohlendieck Kay
Muscle Biology Laboratory, Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland.
Muscles Ligaments Tendons J. 2012 Apr 1;1(4):119-26. Print 2011 Oct.
One of the most striking physiological features of skeletal muscle tissues are their enormous capacity to adapt to changed functional demands. Muscle plasticity has been extensively studied by histological, biochemical, physiological and genetic methods over the last few decades. With the recent emergence of high-throughput and large-scale proteomic techniques, mass spectrometry-based surveys have also been applied to the global analysis of the skeletal muscle protein complement during physiological modifications and pathophysiological alterations. This review outlines and discusses the impact of recent proteomic profiling studies of skeletal muscle transitions, including the effects of chronic electro-stimulation, physical exercise, denervation, disuse atrophy, hypoxia, myotonia, motor neuron disease and age-related fibre type shifting. This includes studies on the human skeletal muscle proteome, animal models of muscle plasticity and major neuromuscular pathologies. The biomedical importance of establishing reliable biomarker signatures for the various molecular and cellular transition phases involved in muscle transformation is critically examined.
骨骼肌组织最显著的生理特征之一是其具有巨大的能力来适应功能需求的变化。在过去几十年中，通过组织学、生物化学、生理学和遗传学方法对肌肉可塑性进行了广泛研究。随着高通量和大规模蛋白质组学技术的近期出现，基于质谱的调查也已应用于对生理修饰和病理生理改变期间骨骼肌蛋白质组的全局分析。本综述概述并讨论了近期骨骼肌转变蛋白质组学分析研究的影响，包括慢性电刺激、体育锻炼、去神经支配、废用性萎缩、缺氧、肌强直、运动神经元疾病和年龄相关纤维类型转变的影响。这包括对人类骨骼肌蛋白质组、肌肉可塑性动物模型和主要神经肌肉疾病的研究。还批判性地审视了为肌肉转化所涉及的各种分子和细胞转变阶段建立可靠生物标志物特征的生物医学重要性。
