Staunton Lisa, O'Connell Kathleen, Ohlendieck Kay
Department of Biology, National University of Ireland, Maynooth, County Kildare, Ireland.
J Aging Res. 2011 Mar 7;2011:908035. doi: 10.4061/2011/908035.
Mitochondria are of central importance for energy generation in skeletal muscles. Expression changes or functional alterations in mitochondrial enzymes play a key role during myogenesis, fibre maturation, and various neuromuscular pathologies, as well as natural fibre aging. Mass spectrometry-based proteomics suggests itself as a convenient large-scale and high-throughput approach to catalogue the mitochondrial protein complement and determine global changes during health and disease. This paper gives a brief overview of the relatively new field of mitochondrial proteomics and discusses the findings from recent proteomic surveys of mitochondrial elements in aged skeletal muscles. Changes in the abundance, biochemical activity, subcellular localization, and/or posttranslational modifications in key mitochondrial enzymes might be useful as novel biomarkers of aging. In the long term, this may advance diagnostic procedures, improve the monitoring of disease progression, help in the testing of side effects due to new drug regimes, and enhance our molecular understanding of age-related muscle degeneration.
线粒体对于骨骼肌的能量生成至关重要。线粒体酶的表达变化或功能改变在肌生成、纤维成熟、各种神经肌肉疾病以及自然纤维老化过程中起着关键作用。基于质谱的蛋白质组学是一种便捷的大规模高通量方法，可用于编目线粒体蛋白质组并确定健康和疾病期间的全局变化。本文简要概述了线粒体蛋白质组学这一相对较新的领域，并讨论了近期对老年骨骼肌线粒体成分进行蛋白质组学调查的结果。关键线粒体酶的丰度、生化活性、亚细胞定位和/或翻译后修饰的变化可能作为衰老的新型生物标志物。从长远来看，这可能会推进诊断程序，改善对疾病进展的监测，有助于测试新药方案的副作用，并增强我们对与年龄相关的肌肉退化的分子理解。
