Department of Sleep Medicine and Metabolic Disorders, Medical University of Lodz, 92-215 Lodz, Poland.
Department of Biochemistry, Medical University of Lodz, 90-419 Lodz, Poland.
Int J Mol Sci. 2024 Sep 27;25(19):10445. doi: 10.3390/ijms251910445.
Deprivation of sleep (DS) and its effects on circadian rhythm gene expression are not well understood despite their influence on various physiological and psychological processes. This study aimed to elucidate the changes in the expression of circadian rhythm genes following a night of sleep and DS. Their correlation with sleep architecture and physical activity was also examined. The study included 81 participants who underwent polysomnography (PSG) and DS with actigraphy. Blood samples were collected after PSG and DS. Expression levels of brain and muscle ARNT-like 1 (BMAL1), circadian locomotor output cycles kaput (CLOCK), neuronal PAS domain protein 2 (NPAS2), period 1 (PER1), cryptochrome 1 (CRY1) and nuclear receptor subfamily 1 group D member 1 (NR1D1) were analyzed using qRT-PCR. DS decreased the expression of CLOCK and BMAL1 while increasing PER1. PER1 expression correlated positively with total sleep time and non-rapid-eye-movement (NREM) sleep duration and negatively with sleep latency, alpha, beta and delta waves in the O1A2 lead. Physical activity during DS showed positive correlations with CLOCK, BMAL1, and CRY1. The findings highlight the role of PER1 in modulating sleep patterns, suggesting potential targets for managing sleep-related disorders. Further research is essential to deepen the understanding of these relationships and their implications.
尽管睡眠剥夺 (DS) 及其对昼夜节律基因表达的影响对各种生理和心理过程都有影响，但人们对其了解甚少。本研究旨在阐明一夜睡眠和 DS 后昼夜节律基因表达的变化，并研究其与睡眠结构和体力活动的相关性。该研究纳入了 81 名参与者，他们接受了多导睡眠图 (PSG) 和活动记录仪监测的 DS。PSG 和 DS 后采集血样。使用 qRT-PCR 分析了脑和肌肉 ARNT 样 1 (BMAL1)、昼夜节律运动输出周期 kaput (CLOCK)、神经元 PAS 域蛋白 2 (NPAS2)、周期 1 (PER1)、隐色素 1 (CRY1) 和核受体亚家族 1 组 D 成员 1 (NR1D1) 的表达水平。DS 降低了 CLOCK 和 BMAL1 的表达，同时增加了 PER1 的表达。PER1 的表达与总睡眠时间和非快速眼动 (NREM) 睡眠时间呈正相关，与睡眠潜伏期、O1A2 导联的 alpha、beta 和 delta 波呈负相关。DS 期间的体力活动与 CLOCK、BMAL1 和 CRY1 呈正相关。研究结果强调了 PER1 在调节睡眠模式中的作用，提示了管理与睡眠相关障碍的潜在靶点。进一步的研究对于加深对这些关系及其影响的理解至关重要。
