微小 RNA181 通过靶向猪繁殖与呼吸综合征病毒（PRRSV）受体 CD163 抑制 PRRSV 感染。
MicroRNA 181 suppresses porcine reproductive and respiratory syndrome virus (PRRSV) infection by targeting PRRSV receptor CD163.
机构信息
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China.
出版信息
J Virol. 2013 Aug;87(15):8808-12. doi: 10.1128/JVI.00718-13. Epub 2013 Jun 5.
Abstract
We previously showed that microRNA 181 (miR-181) can inhibit PRRSV replication by directly targeting its genomic RNA. Here, we report that miR-181 can downregulate the PRRSV receptor CD163 in blood monocytes and porcine alveolar macrophages (PAMs) through targeting the 3' untranslated region (UTR) of CD163 mRNA. Downregulation of CD163 leads to the inhibition of PRRSV entry into PAMs and subsequently suppresses PRRSV infection. Our findings indicate that delivery of miR-181 can be used as antiviral therapy against PRRSV infection.
摘要
我们之前的研究表明，微小 RNA 181（miR-181）可以通过直接靶向其基因组 RNA 来抑制 PRRSV 的复制。在这里，我们报告称，miR-181 可以通过靶向 CD163 mRNA 的 3'非翻译区（UTR）下调血液单核细胞和猪肺泡巨噬细胞（PAMs）中的 PRRSV 受体 CD163。CD163 的下调导致 PRRSV 进入 PAMs 的抑制，从而抑制 PRRSV 感染。我们的研究结果表明，miR-181 的递送可用作针对 PRRSV 感染的抗病毒治疗。
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