伊朗伊斯法罕市自来水和瓶装饮用水中大肠杆菌、沙门氏菌和霍乱弧菌的检测
Detection of Escherichia coli, Salmonella species, and Vibrio cholerae in tap water and bottled drinking water in Isfahan, Iran.
作者信息
Momtaz Hassan, Dehkordi Farhad Safarpoor, Rahimi Ebrahim, Asgarifar Amin
机构信息
Department of Microbiology, College of Veterinary Medicine, ShahreKord Branch, Islamic Azad University, P,O, Box: 166, ShahreKord, Iran.
出版信息
BMC Public Health. 2013 Jun 7;13:556. doi: 10.1186/1471-2458-13-556.
BACKGROUND
The quality of drinking water has an important role in human infection and disease. This study was aimed at comparing polymerase chain reaction and culture in detecting Escherichia coli, Salmonella species and Vibrio cholera in tape water and bottled drinking water in various seasons in Isfahan province, Iran.
METHODS
A total of 448 water samples from tap water and bottled mineral water were taken over 6 months, from July 2010 to December 2010, and after filtration, samples were examined by culture and polymerase chain reaction methods for detection of Escherichia coli, Salmonella species, and Vibrio cholerae.
RESULTS
The culture method showed that 34 (7.58%), 4 (0.89%) and 3 (0.66%) of all 448 water samples were positive for Escherichia coli, Salmonella species, and Vibrio cholera, respectively. The uidA gene from Escherichia coli, IpaB gene from Salmonella species, and epsM gene from Vibrio cholera were detected in 38 (26.38%), 5 (3.47%), and 3 (2.08%) of 144 tap-water samples, respectively. Escherichia coli was detected in 8 (2.63%) of 304 samples of bottled drinking water from 5 companies. The water of southern part of Isfahan and company 5 had the highest prevalence of bacteria. The Escherichia coli water contamination was significantly higher (P < 0.05) in the hot seasons (July-August) than cold (November-December) seasons and in company 5 than other companies. There were significant differences (P < 0.05) for the prevalence of bacteria between the tap waters of southern part and tap waters of central part of Isfahan.
CONCLUSIONS
This study showed that the polymerase chain reaction assays can be an extremely accurate, fast, safe, sensitive and specific approach to monitor drinking water quality from purification facilities and bottled water companies. Also, our study confirmed the presence of Escherichia coli, Salmonella species, and Vibrio cholerae as water-borne pathogens in tap water and bottled drinking water of Isfahan, Iran. The present study showed the important public health problem in Isfahan, Iran.
背景
饮用水质量在人类感染和疾病中起着重要作用。本研究旨在比较聚合酶链反应（PCR）和培养法在检测伊朗伊斯法罕省不同季节自来水和瓶装饮用水中的大肠杆菌、沙门氏菌属和霍乱弧菌方面的效果。
方法
2010年7月至2010年12月的6个月期间，共采集了448份来自自来水和瓶装矿泉水的水样，过滤后，采用培养法和聚合酶链反应法检测大肠杆菌、沙门氏菌属和霍乱弧菌。
结果
培养法显示，在所有448份水样中，分别有34份（7.58%）、4份（0.89%）和3份（0.66%）的水样检测出大肠杆菌、沙门氏菌属和霍乱弧菌呈阳性。在144份自来水样本中，分别有38份（26.38%）、5份（3.47%）和3份（2.08%）检测出大肠杆菌的uidA基因、沙门氏菌属的IpaB基因和霍乱弧菌的epsM基因。在来自5家公司的304份瓶装饮用水样本中，有8份（2.63%）检测出大肠杆菌。伊斯法罕南部地区的水以及5号公司的水细菌污染率最高。炎热季节（7 - 8月）的大肠杆菌水污染率显著高于寒冷季节（11 - 12月）（P < 0.05），5号公司的水污染率显著高于其他公司。伊斯法罕南部地区的自来水与中部地区的自来水在细菌污染率上存在显著差异（P < 0.05）。
结论
本研究表明，聚合酶链反应检测法是一种极其准确、快速、安全、灵敏且特异的方法，可用于监测净化设施和瓶装水公司的饮用水质量。此外，我们的研究证实了伊朗伊斯法罕的自来水和瓶装饮用水中存在作为水源性病原体的大肠杆菌、沙门氏菌属和霍乱弧菌。本研究揭示了伊朗伊斯法罕存在的重要公共卫生问题。
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