Department of Biotechnology, Yonsei University, Seoul, Republic of Korea.
J Control Release. 2013 Sep 28;170(3):430-6. doi: 10.1016/j.jconrel.2013.05.026. Epub 2013 Jun 3.
The microneedle-mediated drug delivery system has been developed to provide painless self-administration of drugs in a patient-friendly manner. Current dissolving microneedle fabrication methods, however, require harsh conditions for biological drugs and also have problems standardizing the drug dose. Here, we suggested the droplet-born air blowing (DAB) method, which provides gentle (4-25 °C) and fast (≤10min) microneedle fabrication conditions without drug loss. The amount of drug in the microneedle can be controlled by the pressure and time of droplet dispenser and the air blowing shapes this droplet to the microneedle, providing a force sufficient to penetrate skin. Also, the introduction of a base structure of two layered DAB-microneedle could provide complete drug delivery without wasting of drug. The DAB-based insulin loaded microneedle shows similar bioavailability (96.6±2.4%) and down regulation of glucose level compared with subcutaneous injection. We anticipate that DAB described herein will be suitable to design dissolving microneedles for use in biological drug delivery to patients.
微针介导的药物输送系统已经被开发出来，以便以患者友好的方式提供无痛的药物自我给药。然而，目前的溶解微针制造方法需要苛刻的生物药物条件，并且在标准化药物剂量方面也存在问题。在这里，我们提出了液滴吹气（DAB）方法，该方法提供温和的（4-25°C）和快速的（≤10 分钟）微针制造条件，而不会导致药物损失。微针中的药物量可以通过液滴分配器的压力和时间来控制，吹气将液滴吹成微针形状，提供足够的力穿透皮肤。此外，引入两层 DAB-微针的基础结构可以提供完全的药物输送，而不会浪费药物。基于 DAB 的胰岛素负载微针显示出与皮下注射相似的生物利用度（96.6±2.4%）和葡萄糖水平下调。我们预计，本文所述的 DAB 将适合用于设计用于向患者输送生物药物的溶解微针。
