Ganjali Amin, Sotoudeh Amir, Jahanshahi Amirali, Takhtfooladi Mohammad Ashrafzadeh, Bazzazan Ali, Roodbari Nasim, Harati Maryam Pourramezani
Faculty of Experimental Science, Kahnooj Branch, Islamic Azad University, Kerman, Iran.
Acta Cir Bras. 2013 Jun;28(6):407-11. doi: 10.1590/s0102-86502013000600001.
To investigate if the methanolic extract of the Otostegia persica can accelerating healing process of burn wound because of its anti-inflammatory and antioxidant effects.
Forty eight male Wistar rats were randomized into three study groups of 16 rats each. Burn wounds were created on dorsal part of shaved rats using a metal rod. In group I the burn wound was left without any treatment. Group was treated with topical silver sulfadiazine pomade. In group III, ointment containing the OP extract was administered. Skin biopsies were harvested from burn area on the 3rd, 5th, 14th and 21st days after burn and examined histologically.
Re-epithelialization in the control group and in group II was lower than in group III. Re-epithelialization in groups II and III was significantly different from that in the control group. On the 5th day of the experiment, we assessed lower inflammation in the burn area compared to control group. This means that the inflammation was suppressed by methanolic extract of OP. From day 5 to 14; the fibroblast proliferation peaked and was associated with increased collagen accumulation. It was obvious that angiogenesis improved more in the groups II and III, which facilitated re-epithelialisation.
Methanolic extract of Otostegia persica exhibited significant healing activity when topically applied on rats. OP is an effective treatment for saving the burn site.
研究波斯耳草甲醇提取物是否因其抗炎和抗氧化作用而能加速烧伤创面的愈合过程。
将48只雄性Wistar大鼠随机分为三个研究组，每组16只。用金属棒在剃毛大鼠的背部制造烧伤创面。第一组烧伤创面不做任何处理。第二组用磺胺嘧啶银软膏局部治疗。第三组给予含波斯耳草提取物的软膏。在烧伤后第3天、第5天、第14天和第21天从烧伤部位采集皮肤活检组织并进行组织学检查。
对照组和第二组的再上皮化低于第三组。第二组和第三组的再上皮化与对照组有显著差异。在实验的第5天，我们评估发现与对照组相比，烧伤部位的炎症较轻。这意味着波斯耳草甲醇提取物抑制了炎症。从第5天到第14天，成纤维细胞增殖达到峰值并伴有胶原积累增加。很明显，第二组和第三组的血管生成改善得更多，这促进了再上皮化。
波斯耳草甲醇提取物局部应用于大鼠时表现出显著的愈合活性。波斯耳草是治疗烧伤创面的有效药物。
