Department of Microbiology and Research Center for Medical Sciences, Chung-Ang University College of Medicine, Seoul, South Korea.
Arch Virol. 2013 Nov;158(11):2385-93. doi: 10.1007/s00705-013-1720-9. Epub 2013 Jun 7.
A rare rotavirus, RVA/Human-wt/KOR/CAU12-2/2012/G11P[25], was isolated from a 16-year-old female with fever and diarrhea during the 2012 rotavirus surveillance in South Korea using a cell culture system, and its full genome sequence was determined and analyzed. Strain CAU12-2 exhibited a G11-P[25]-I12-R1-C1-M1-A1-N1-T1-E1-H1 genotype constellation. Phylogenetic analysis of this strain revealed that it is a human-porcine reassortant of two distant relatives of the G11 strains circulating in the world. The VP7 and VP4 genes are most closely related to those of human G11P[25] viruses (Dhaka6, KTM368, and N-38 strains) identified in South Asia, whereas the VP1 gene originated from a porcine G11P[7] virus (YM strain) that was identified in South America. The VP6 gene was found to belong to the new genotype I12. This study indicates that the G11-P[25]-I12 genotype was introduced into the South Korean population by interspecies transmissions of human and animal rotaviruses, followed by multiple reassortment events.
一株罕见的轮状病毒，RVA/Human-wt/KOR/CAU12-2/2012/G11P[25]，于 2012 年在韩国进行的轮状病毒监测中，从一名出现发热和腹泻的 16 岁女性患者中分离得到，该病毒采用细胞培养系统分离，并确定和分析了其全基因组序列。菌株 CAU12-2 表现出 G11-P[25]-I12-R1-C1-M1-A1-N1-T1-E1-H1 基因型组合。该毒株的系统进化分析表明，它是在世界范围内流行的 G11 株的两个远亲之间的人-猪重组体。VP7 和 VP4 基因与在南亚鉴定的人 G11P[25]病毒（达卡 6、KTM368 和 N-38 株）最为密切相关，而 VP1 基因来源于在南美洲鉴定的猪 G11P[7]病毒（YM 株）。VP6 基因被发现属于新基因型 I12。本研究表明，G11-P[25]-I12 基因型通过人源和动物源轮状病毒的种间传播传入韩国人群，并随后发生了多次重配事件。
