Bianchi Luciana, Ribeiro Ana Paula Dias, Carrilho Marcela Rocha de Oliveira, Pashley David H, de Souza Costa Carlos Alberto, Hebling Josimeri
Department of Pediatric Dentistry and Orthodontics, Araraquara School of Dentistry, UNESP-Univ Estadual Paulista, Araraquara, Sao Paulo, Brazil.
J Biomed Mater Res B Appl Biomater. 2013 Nov;101(8):1498-507. doi: 10.1002/jbm.b.32971. Epub 2013 Jun 7.
This study evaluated the cytotoxicity of experimental adhesive systems (EASs) on odontoblast-like cells. Paper discs (n = 132) were impregnated with 10 µL of each EAS-R1, R2, R3, R4, and R5 (in an ascending order of hydrophilicity), followed by photoactivation. R1 and R2 are nonsolvated hydrophobic blends, R3 represents a simplified etch-and-rinse adhesive system, and R4 and R5 represent simplified self-etch adhesive systems. Discs were immersed in Dulbecco's modified Eagle's medium for 24 h to obtain eluates applied on MDPC-23 cell cultures. No material was applied on discs used as control (R0). Cell viability [3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay], total protein (TP) production, alkaline phosphatase (ALP) activity, type of cell death, and degree of monomer conversion Fourier transform infrared (%DC-FTIR) were evaluated. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (α = 0.05). Considering R0 (control) as having 100% of cell viability, R1, R2, R3, R4, and R5 reduced the metabolic activity of cells by 36.4, 3.1, 0.2, 21.5, and 65.7%, respectively, but only R1 and R5 differed from R0. Comparing with R0, lower TP production was observed for R1, R4, and R5, while ALP activity decreased for R1 and R5. Necrotic cell death was predominant for all EASs, but only R1, R4, and R5 differed from R0. Only R5 presented a different apoptotic cell death ratio from R0. R1 presented the lowest %DC (ca. 37%), whereas R4 and R5 presented the highest (ca. 56%). In conclusion, R2 and R3 were not toxic to the MDPC-23 cells, suggesting that the degree of hydrophilicity or %DC of the EASs alone were not responsible for their cytopathic effects.
本研究评估了实验性黏结系统（EASs）对成牙本质细胞样细胞的细胞毒性。将圆纸片（n = 132）分别用10 μL的每种EAS-R1、R2、R3、R4和R5（按亲水性升序排列）浸渍，然后进行光固化。R1和R2是未溶剂化的疏水混合物，R3代表一种简化的酸蚀冲洗黏结系统，R4和R5代表简化的自酸蚀黏结系统。将圆纸片浸入杜氏改良 Eagle 培养基中24小时以获得洗脱液，用于MDPC-23细胞培养。用作对照的圆纸片（R0）不施加任何材料。评估细胞活力[3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑法]、总蛋白（TP）产量、碱性磷酸酶（ALP）活性、细胞死亡类型和单体转化率傅里叶变换红外光谱（%DC-FTIR）。数据采用 Kruskal-Wallis 检验和 Mann-Whitney 检验进行分析（α = 0.05）。将R0（对照）视为细胞活力为100%，R1、R2、R3、R4和R5分别使细胞的代谢活性降低了36.4%、3.1%、0.2%、21.5%和65.7%，但只有R1和R5与R0不同。与R0相比，R1、R4和R5的TP产量较低，而R1和R5的ALP活性降低。所有EASs均以坏死性细胞死亡为主，但只有R1、R4和R5与R0不同。只有R5的凋亡细胞死亡率与R0不同。R1的%DC最低（约37%），而R4和R5的%DC最高（约56%）。总之，R2和R3对MDPC-23细胞无毒，这表明单独的EASs亲水性程度或%DC并非其细胞病变效应的原因。
