Wawrzynkiewicz Adam, Rozpedek-Kaminska Wioletta, Galita Grzegorz, Lukomska-Szymanska Monika, Lapinska Barbara, Sokolowski Jerzy, Majsterek Ireneusz
Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-419 Lodz, Poland.
Department of General Dentistry, Medical University of Lodz, 92-213 Lodz, Poland.
Polymers (Basel). 2021 Aug 10;13(16):2653. doi: 10.3390/polym13162653.
There is no consensus in the literature regarding the potential toxicity of universal dental adhesives (UDA). Being used in close proximity to the pulp, their biocompatibility should be an important factor in dental research. The aim of the present study was to evaluate the biocompatibility of UDA in an in vitro model. The study was performed using a monocyte/macrophage peripheral blood SC cell line (ATCC CRL-9855) on four specific UDA, namely: All-Bond Universal (Bisco); CLEARFIL Universal Bond Quick (Kuraray); G-Premio BOND (GC); Single Bond Universal (3M ESPE). The cytotoxicity of the investigated UDA was measured using the XTT colorimetric assay. The genotoxicity of the analyzed compounds was evaluated using an alkaline version of the comet assay. Furthermore, flow cytometry (FC) apoptosis detection was performed using the FITC Annexin V Apoptosis Detection Kit I. FC cell-cycle arrest assessment was performed using propidium iodide staining. The study observed significant differences in the toxicity of the UDA that were tested, as G-Premio BOND showed significant in vitro toxicity in all of the tests performed, while All-Bond Universal, CLEARFIL Universal Bond Quick and Single Bond Universal did not present any significant toxic effects toward SC cell line. The in vitro toxicity of UDA should be taken into consideration prior to in vivo and clinical studies. The flow cytometry could improve the accuracy of dental materials research and should be incorporated into the standardization criteria.
关于通用牙科粘合剂（UDA）的潜在毒性，文献中尚无共识。由于它们在牙髓附近使用，其生物相容性应是牙科研究中的一个重要因素。本研究的目的是在体外模型中评估UDA的生物相容性。该研究使用单核细胞/巨噬细胞外周血SC细胞系（ATCC CRL - 9855）对四种特定的UDA进行，即：全粘结通用型（Bisco）；可乐丽菲露通用粘结快速型（可乐丽）；G - 超粘结剂（GC）；单键通用型（3M ESPE）。使用XTT比色法测量所研究UDA的细胞毒性。使用彗星试验的碱性版本评估分析化合物的遗传毒性。此外，使用FITC膜联蛋白V凋亡检测试剂盒I进行流式细胞术（FC）凋亡检测。使用碘化丙啶染色进行FC细胞周期阻滞评估。研究观察到所测试的UDA在毒性方面存在显著差异，因为G - 超粘结剂在所有进行的测试中均显示出显著的体外毒性，而全粘结通用型、可乐丽菲露通用粘结快速型和单键通用型对SC细胞系未呈现任何显著的毒性作用。在进行体内和临床研究之前，应考虑UDA的体外毒性。流式细胞术可以提高牙科材料研究的准确性，应纳入标准化标准。
