Institute of Food Research, Norwich Research Park, Norwich, NR4 7UA, UK.
J Sci Food Agric. 2013 Nov;93(14):3574-80. doi: 10.1002/jsfa.6251. Epub 2013 Jul 5.
There is increasing evidence that consumption of plant bioactives such as polyphenols and glucosinolates reduces cardiovascular disease risk and improves endothelial function. In the Black Sea area, a number of plants are consumed alone and as ingredients in traditional foods, and dill, nettle, kale, Sideritis and persimmon were identified as bioactive-rich traditional food plants. The present study investigated the effects of plant extracts on cellular markers of endothelial function (eNOS activation and expression and ET-1 secretion).
Treatment of human umbilical vein endothelial cells with persimmon extract significantly increased Akt and eNOS phosphorylation and nitric oxide metabolites and significantly decreased secretion of ET-1 to the media after 24 h compared with a vehicle control (all P < 0.01). None of the other plant extracts significantly altered any markers of endothelial function.
These findings suggest that persimmon fruit contains bioactives that can improve endothelial function via activation of eNOS and reduction in ET-1 secretion, but that dill, kale, Sideritis and nettle do not.
越来越多的证据表明，植物生物活性物质（如多酚和硫代葡萄糖苷）的摄入可以降低心血管疾病风险并改善血管内皮功能。在黑海地区，有许多植物被单独食用或作为传统食品的成分，其中莳萝、荨麻、羽衣甘蓝、山萝卜和柿子被确定为富含生物活性的传统食物植物。本研究调查了植物提取物对血管内皮功能的细胞标志物（eNOS 激活和表达以及 ET-1 分泌）的影响。
与载体对照组相比，24 小时后，柿子提取物处理人脐静脉内皮细胞可显著增加 Akt 和 eNOS 的磷酸化以及一氧化氮代谢物的产生，并显著降低 ET-1 向培养基中的分泌（均 P < 0.01）。其他植物提取物均未显著改变任何血管内皮功能标志物。
这些发现表明，柿子果实中含有生物活性物质，可通过激活 eNOS 和减少 ET-1 分泌来改善血管内皮功能，但莳萝、羽衣甘蓝、山萝卜和荨麻则没有。
