Yang Jinwei, Dong Yitong, Hu Lixinyi, Wang Weihai, Li Yajun, Wang Shujie, Wang Chunsheng
College of Life Science, Northeast Forestry University, Harbin 150040, China.
State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Biology (Basel). 2024 Jul 22;13(7):551. doi: 10.3390/biology13070551.
Mesenchymal stem cells (MSCs) possess significant differentiation potential, making them highly promising in medicine and immunotherapy due to their regenerative capabilities and exosome secretion. However, challenges such as limited cell divisions and complex testing hinder large-scale MSC production. In this study, we successfully established an immortalized MSC line by transfecting the human telomerase reverse transcriptase (TERT) gene into MSCs isolated from pregnant sheep umbilical cords. This approach effectively inhibits cell senescence and promotes cell proliferation, enabling the generation of umbilical cord mesenchymal stem cells (UCMSCs) on a larger scale. Our findings demonstrate that these transfected TERT-UCMSCs exhibit enhanced proliferative capacity and a reduced aging rate compared to regular UCMSCs while maintaining their stemness without tumorigenicity concerns. Consequently, they hold great potential for medical applications requiring large quantities of functional MSCs.
间充质干细胞（MSCs）具有显著的分化潜能，由于其再生能力和外泌体分泌，使其在医学和免疫治疗方面具有很高的应用前景。然而，诸如有限的细胞分裂和复杂的检测等挑战阻碍了大规模的间充质干细胞生产。在本研究中，我们通过将人端粒酶逆转录酶（TERT）基因转染到从怀孕绵羊脐带分离的间充质干细胞中，成功建立了永生化的间充质干细胞系。这种方法有效地抑制了细胞衰老并促进了细胞增殖，从而能够大规模地生成脐带间充质干细胞（UCMSCs）。我们的研究结果表明，与常规脐带间充质干细胞相比，这些转染了TERT的脐带间充质干细胞表现出增强的增殖能力和降低的衰老率，同时保持其干性，不存在致瘤性问题。因此，它们在需要大量功能性间充质干细胞的医学应用中具有巨大潜力。
