Department of Neurobiology & Anatomy, University of Rochester, 601 Elmwood Avenue, Box 603, Rochester, NY 14642, USA.
Curr Protein Pept Sci. 2013 Aug;14(5):407-15. doi: 10.2174/13892037113149990057.
The receptor for calcitonin gene-related peptide (CGRP) and adrenomedullin (AM) requires an intracellular peripheral membrane protein named CGRP-receptor component protein (RCP) for signaling. RCP is required for CGRP and AM receptor signaling, and it has recently been discovered that RCP enables signaling by binding directly to the receptor. RCP is present in most immortalized cell lines, but in vivo RCP expression is limited to specific subsets of cells, usually co-localizing with CGRP-containing neurons. RCP protein expression correlates with CGRP efficacy in vivo, suggesting that RCP regulates CGRP signaling in vivo as it does in cell culture. RCP is usually identified in cytoplasm or membranes of cells, but recently has been observed in nucleus of neurons, suggesting an additional transcriptional role for RCP in cell function. Together, these data support an essential role for RCP in CGRP and AM receptor function, in which RCP expression enhances signaling of the CGRP or AM receptor, and therefore increases the efficacy of CGRP and AM in vivo.
降钙素基因相关肽（CGRP）和肾上腺髓质素（AM）的受体需要一种细胞内外周膜蛋白，称为 CGRP 受体成分蛋白（RCP），才能进行信号传递。RCP 是 CGRP 和 AM 受体信号传递所必需的，最近发现 RCP 通过直接与受体结合来实现信号传递。RCP 存在于大多数永生化细胞系中，但在体内，RCP 的表达仅限于特定的细胞亚群，通常与含有 CGRP 的神经元共定位。RCP 蛋白表达与体内 CGRP 的功效相关，表明 RCP 像在细胞培养中一样调节体内的 CGRP 信号传递。RCP 通常在细胞的细胞质或膜中被识别，但最近在神经元的核中也观察到了 RCP，这表明 RCP 在细胞功能中具有额外的转录作用。这些数据共同支持 RCP 在 CGRP 和 AM 受体功能中的重要作用，其中 RCP 的表达增强了 CGRP 或 AM 受体的信号传递，从而增加了体内 CGRP 和 AM 的功效。
