Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, KSA.
Technol Cancer Res Treat. 2013 Dec;12(6):559-63. doi: 10.7785/tcrt.2012.500345. Epub 2013 Jun 6.
Pathological condition of malignant tissue could be analyzed by spectral domain or time domain spectroscopy, the two being the complementary to each other in optical biopsy (OB) of cancer. This paper reports results of time resolved emission spectroscopy (TRS) of 24 excised tissue samples of breast and prostate (normal control = 12; benign = 4; malignant = 8), employing a 390 nm, 100 fs, Ti-Sapphire laser pulses.The fluorescence decay times were measured using streak camera and the resultant data were fitted for single and bi-exponential decays with reliability of 97%. Our results show the distinct difference between normal, benign and malignant tissues mostly due to the emission spectra of Nicotinamide Adenine Dinucleotide (NADH), Flavin Mononucleotide (FAD) and also due to the heterogeneity of micro environments associated with the diseased tissues. In this short report, fit is also shown that TRS of breast tissues are similar to those of prostate tissues.
通过光谱域或时域光谱分析恶性组织的病理状态，这两种方法在癌症的光学活检中是互补的。本文报告了采用 390nm、100fs、Ti 蓝宝石激光脉冲对 24 个切除的乳腺和前列腺组织样本（正常对照=12；良性=4；恶性=8）进行时间分辨发射光谱（TRS）的结果。使用条纹相机测量荧光衰减时间，并用 97%的可靠性对单指数和双指数衰减进行拟合。我们的结果表明，正常、良性和恶性组织之间存在明显差异，这主要是由于烟酰胺腺嘌呤二核苷酸（NADH）、黄素单核苷酸（FAD）的发射光谱以及与疾病组织相关的微环境的异质性所致。在这个简短的报告中，还表明乳腺组织的 TRS 与前列腺组织的 TRS 相似。
