Damha M J, Giannaris P A, Zabarylo S V
Department of Chemistry, Erindale College, University of Toronto, Mississauga, Ontario, Canada.
Nucleic Acids Res. 1990 Jul 11;18(13):3813-21. doi: 10.1093/nar/18.13.3813.
A simplified and economical method for the attachment of 2'-deoxyribo, ribo and arabinonucleosides onto long-chain alkylamidopropanoic acid controlled-pore glass (LCAAP-CPG, P-3) is described. In this procedure, 5'-O-tritylated nucleosides are coupled directly to LCAAP-CPG in excellent yields using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (DEC) as coupling reagent. The conventional and time-consuming preparation of nucleoside-3'-O-succinates is no longer required.
描述了一种将2'-脱氧核糖核苷、核糖核苷和阿拉伯核苷连接到长链烷基酰胺基丙酸可控孔玻璃（LCAAP-CPG，P-3）上的简化且经济的方法。在此方法中，使用1-(3-二甲基氨基丙基)-3-乙基碳二亚胺（DEC）作为偶联试剂，5'-O-三苯甲基化核苷以优异的产率直接与LCAAP-CPG偶联。不再需要常规且耗时的核苷-3'-O-琥珀酸酯的制备。
