Laboratorio de Venómica y Proteinómica Estructural, Instituto de Biomedicina de Valencia, C.S.I.C., Valencia, Spain.
J Proteomics. 2013 Aug 26;89:112-23. doi: 10.1016/j.jprot.2013.05.028. Epub 2013 Jun 4.
We report the proteomic analysis of the Atlantic bushmaster, Lachesis muta rhombeata, from Brazil. Along with previous characterization of the venom proteomes of L. stenophrys (Costa Rica), L. melanocephala (Costa Rica), L. acrochorda (Colombia), and L. muta muta (Bolivia), the present study provides the first overview of the composition and distribution of venom proteins across this wide-ranging genus, and highlights the remarkable similar compositional and pharmacological profiles across Lachesis venoms. The paraspecificity of two antivenoms, produced at Instituto Vital Brazil (Brazil) and Instituto Clodomiro Picado (Costa Rica) using different conspecific taxa in the immunization mixtures, was assessed using genus-wide comparative antivenomics. This study confirms that the proteomic similarity among Lachesis sp. venoms is mirrored in their high immunological conservation across the genus. The clinical and therapeutic consequences of genus-wide venomics and antivenomics investigations of Lachesis venoms are discussed.
The proteomics characterization of L. m. rhombeata venom completes the overview of Lachesis venom proteomes and confirms the remarkable toxin profile conservation across the five clades of this wide-ranging genus. Genus-wide antivenomics showed that two antivenoms, produced against L. stenophrys or L. m. rhombeata, exhibit paraspecificity towards all other congeneric venoms. Our venomics study shows that, despite the broad geographic distribution of the genus, monospecific antivenoms may achieve clinical coverage for any Lachesis sp. envenoming.
我们报告了巴西大西洋响尾蛇（Lachesis muta rhombeata）的蛋白质组分析。与之前对 L. stenophrys（哥斯达黎加）、L. melanocephala（哥斯达黎加）、L. acrochorda（哥伦比亚）和 L. muta muta（玻利维亚）毒液蛋白质组的表征一起，本研究首次概述了广泛分布的毒液蛋白质的组成和分布，并强调了拉奇西斯毒液在组成和药理学特征上的显著相似性。使用不同的同种毒液在免疫混合物中产生的两种抗蛇毒血清，即巴西的 Instituto Vital Brazil 和哥斯达黎加的 Instituto Clodomiro Picado，其种间特异性使用基于属的比较抗蛇毒血清学进行了评估。这项研究证实了拉奇西斯毒液之间的蛋白质组相似性反映了它们在整个属中高度的免疫保守性。讨论了拉奇西斯毒液的属范围毒液组学和抗蛇毒血清学研究的临床和治疗意义。
L. m. rhombeata 毒液的蛋白质组学特征完成了拉奇西斯毒液蛋白质组的概述，并证实了五个广泛分布的属中显著的毒素谱保守性。基于属的抗蛇毒血清学表明，两种抗蛇毒血清，针对 L. stenophrys 或 L. m. rhombeata 产生，对所有其他同种毒液都表现出种间特异性。我们的毒液组学研究表明，尽管该属的地理分布广泛，但单种抗蛇毒血清可能对任何拉奇西斯蛇毒中毒都能实现临床覆盖。
