Key Laboratory of Animal Models and Human Disease Mechanisms, Key Laboratory of Bioactive Peptides of Yunnan Province, Engineering Laboratory of Bioactive Peptides, The National & Local Joint Engineering Center of Natural bioactive Peptides, Kunming Institute of Zoology-The Chinese University of Hong Kong (KIZ-CUHK) Joint Laboratory of Bioresources and Molecular Research in Common Diseases, National Resource Center for Non-Human Primates, Kunming Primate Research Center, and National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, China.
Front Immunol. 2021 Nov 9;12:775678. doi: 10.3389/fimmu.2021.775678. eCollection 2021.
As said by former United Nations Secretary-General Kofi Annan, "Snakebite is the most important tropical disease you've never heard of." Listed as a priority neglected tropical disease by the World Health Organization, snakebite envenoming (SBE) kills in excess of 125,000 people per year. However, due to the complexity and overlap of snake venom compositions, few reliable venom diagnostic methods for genus-/species-specific identification, which is crucial for successful SBE therapy, are available. Here, we develop a strategy to select and prepare genus-specific snake venom antibodies, which allows rapid and efficient clinical diagnosis of snakebite. Multi-omics approaches are used to choose candidate antigens from snake venoms and identify genus-specific antigenic epitope peptide fragments (GSAEPs) with ideal immunogenicity, specificity, and spatial accessibility. Double-antibody sandwich ELISA kit was established by matching a polyclonal antibody against a natural antigen and a monoclonal antibody that was prepared by natural protein as antigen and can specifically target the GSAEPs. The kit shows the ability to accurately identify venoms from similar genera of and with a detection limit of 6.25 ng/ml on the snake venoms and a little cross-reaction, thus proving high feasibility and applicability.
正如联合国前秘书长科菲·安南所说：“蛇咬伤是你从未听说过的最重要的热带病。”蛇咬伤（SBE）被世界卫生组织列为重点被忽视的热带病，每年导致超过 125,000 人死亡。然而，由于蛇毒成分的复杂性和重叠性，很少有可靠的用于属/种特异性鉴定的毒液诊断方法，这对成功治疗 SBE 至关重要。在这里，我们开发了一种选择和制备属特异性蛇毒抗体的策略，这使得蛇咬伤的临床诊断能够快速而有效地进行。多组学方法用于从蛇毒液中选择候选抗原，并鉴定具有理想免疫原性、特异性和空间可及性的属特异性抗原表位肽片段（GSAEPs）。通过匹配针对天然抗原的多克隆抗体和由天然蛋白作为抗原制备的可特异性靶向 GSAEPs 的单克隆抗体，建立了双抗体夹心 ELISA 试剂盒。该试剂盒能够准确识别来自 和 相似属的毒液，对蛇毒液的检测限为 6.25ng/ml，交叉反应较小，因此证明了其高可行性和适用性。
