College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, People's Republic of China.
Luminescence. 2013 Mar-Apr;28(2):176-82. doi: 10.1002/bio.2359. Epub 2012 Apr 3.
CdS nanoparticles (CdS NPs) of different sizes were synthesized by the citrate reduction method. It was found that CdS NPs could enhance the chemiluminescence (CL) of the luminol-potassium ferricyanide system and baicalin could inhibit CdS NPs-enhanced luminol-potassium ferricyanide CL signals in alkaline solution. Based on this inhibition, a flow-injection CL method was established for determination of baicalin in pharmaceutical preparations and human urine samples. Under optimized conditions, the linear range for determination of baicalin was 5.0 x 10(-6) to 1.0 x 10(-3) g/L. The detection limit at a signal-to-noise ratio of 3 was 1.7 x 10(-6) g/L. CL spectra, UV-visible spectra and transmission electron microscopy (TEM) were used to investigate the CL mechanism. The method described is simple, selective and obviates the need of extensive sample pretreatment.
CdS 纳米粒子（CdS NPs）通过柠檬酸盐还原法合成。研究发现，CdS NPs 能够增强鲁米诺-铁氰化钾体系的化学发光（CL），并且黄芩苷可以抑制碱性溶液中 CdS NPs 增强的鲁米诺-铁氰化钾 CL 信号。基于这种抑制作用，建立了一种用于测定药物制剂和人尿样中黄芩苷的流动注射 CL 法。在优化条件下，测定黄芩苷的线性范围为 5.0×10^-6 至 1.0×10^-3 g/L。信噪比为 3 时的检测限为 1.7×10^-6 g/L。CL 光谱、紫外-可见光谱和透射电子显微镜（TEM）用于研究 CL 机制。该方法简单、选择性好，无需进行广泛的样品预处理。
