Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Department of Bioengineering, Instituto Superior Técnico, Technical University of Lisbon, Av. Rovisco Pais, Lisbon 1049-001, Portugal.
Biotechnol Adv. 2013 Dec;31(8):1374-85. doi: 10.1016/j.biotechadv.2013.05.009. Epub 2013 Jun 6.
Magnetic separations are probably one of the most versatile separation processes in biotechnology as they are able to purify cells, viruses, proteins and nucleic acids directly from crude samples. The fast and gentle process in combination with its easy scale-up and automation provide unique advantages over other separation techniques. In the midst of this process are the magnetic adsorbents tailored for the envisioned target and whose complex synthesis spans over multiple fields of science. In this context, this article reviews both the synthesis and tailoring of magnetic adsorbents for bioseparations as well as their ultimate application.
磁分离可能是生物技术中最通用的分离方法之一，因为它能够直接从粗样品中纯化细胞、病毒、蛋白质和核酸。与其他分离技术相比，该快速温和的过程与易于放大和自动化相结合提供了独特的优势。在这个过程中，有针对预期目标定制的磁性吸附剂，其复杂的合成跨越了多个科学领域。在这种情况下，本文综述了用于生物分离的磁性吸附剂的合成和定制及其最终应用。
