Department of Chemistry, College of Sciences, Shanghai University, Shanghai, P. R. China.
CAS Key Laboratory of Magnetic Materials and Devices & Division of Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, P. R. China.
Electrophoresis. 2019 Aug;40(16-17):2011-2028. doi: 10.1002/elps.201800401. Epub 2019 Feb 27.
As a result of their advantages for superparamagnetic properties, good biocompatibility, and high binding capacity, functionalized magnetic materials became widely popular over the past couple of decades, being applied on large scale in various processes of sample preparation for biomedicine. In this work, we perform an in-depth review on the current progress in the field of magnetic bead separation, discussing in detail the physical basis of this process, various synthesis methods and surface modification strategies. We place special focus of attention as well on the latest applications of magnetic polymer microspheres in cell separation, protein purification, immobilized enzyme, nucleic acid separation, and extraction of bioactive compounds with low molecular weight. Existing problems are highlighted and possible trends of magnetic separation techniques for biomedicine in the future are proposed.
由于其超顺磁性、良好的生物相容性和高结合能力等优势，功能化磁性材料在过去几十年中得到了广泛的应用，并在生物医学样品制备的各个过程中大规模应用。在这项工作中，我们对磁珠分离领域的当前进展进行了深入的回顾，详细讨论了该过程的物理基础、各种合成方法和表面修饰策略。我们特别关注磁性聚合物微球在细胞分离、蛋白质纯化、固定化酶、核酸分离以及低分子量生物活性化合物提取中的最新应用。突出了存在的问题，并提出了未来生物医学中磁分离技术的可能发展趋势。
