Division of Allergy, Pulmonary and Critical Care Medicine, Vanderbilt School of Medicine, Nashville, TN, USA.
Nanomedicine. 2013 Nov;9(8):1235-44. doi: 10.1016/j.nano.2013.05.010. Epub 2013 Jun 6.
Polylysine (PL) has been used to facilitate dendritic cell (DC) uptake of super paramagnetic iron oxide (SPIO) nanoparticles for use in magnetic resonance imaging (MRI). In this work, we examined the effect of PL on cell toxicity and induction of cell maturation as manifested by the up-regulation of surface molecules. We found that PL became toxic to bone marrow-derived DCs (BMDCs) at the 10 μg/ml threshold. Incubation of BMDCs with 20 μg/ml of PL for 1h resulted in approximately 90% cell death. However, addition of SPIO nanoparticles rescued DCs from PL-induced death as the combination of SPIO with PL did not cause cytotoxicity until the PL concentration was 1000 μg/ml. Prolonged exposure to PL induced BMDC maturation as noted by the expression of surface molecules such as MHC class II, CD40, CCR7 and CD86. However, the combination of SPIO and PL did not induce BMDC maturation at 1h. However prolonged exposure to SPIO nanoparticles induced CD40 expression and protein expression of TNFα and KC. The data suggest that the use of PL to enhance the labeling of DCs with SPIO nanoparticles is a dedicated work. Appropriate calibration of the incubation time and concentrations of PL and SPIO nanoparticles is crucial to the development of MRI technology for noninvasive imaging of DCs in vivo.
The authors of this study present detailed data on toxicity and efficiency of polylysine-facilitated uptake of USPIO-s by dendritic cells for cell-specific MR imaging.
多聚赖氨酸（PL）已被用于促进树突状细胞（DC）摄取超顺磁性氧化铁（SPIO）纳米颗粒，以用于磁共振成像（MRI）。在这项工作中，我们研究了 PL 对细胞毒性和细胞成熟诱导的影响，表现为表面分子的上调。我们发现，PL 在 10μg/ml 的阈值时对骨髓来源的树突状细胞（BMDC）变得有毒。将 BMDC 与 20μg/ml 的 PL 孵育 1h 导致大约 90%的细胞死亡。然而，SPIO 纳米颗粒的加入挽救了 DC 免受 PL 诱导的死亡，因为 SPIO 与 PL 的组合直到 PL 浓度达到 1000μg/ml 才会引起细胞毒性。PL 的长时间暴露诱导 BMDC 成熟，如表面分子 MHC 类 II、CD40、CCR7 和 CD86 的表达。然而，SPIO 和 PL 的组合在 1h 内不会诱导 BMDC 成熟。然而，长时间暴露于 SPIO 纳米颗粒诱导 CD40 表达和 TNFα 和 KC 的蛋白表达。数据表明，使用 PL 增强 SPIO 纳米颗粒标记 DC 是一项专门的工作。PL 和 SPIO 纳米颗粒的孵育时间和浓度的适当校准对于开发用于体内非侵入性成像 DC 的 MRI 技术至关重要。
该研究的作者提供了有关多聚赖氨酸促进 USPIO-s 摄取树突状细胞的毒性和效率的详细数据，用于细胞特异性磁共振成像。
