Biomedical Sciences Department, Defence Science and Technology Laboratory (DSTL), Porton Down, Salisbury, Wiltshire SP4 0JQ, UK.
J Virol Methods. 2013 Nov;193(2):565-71. doi: 10.1016/j.jviromet.2013.05.015. Epub 2013 Jun 5.
Two common methods of quantifying filovirus infectivity, a plaque assay and 50% cell culture infectious dose (TCID50) endpoint dilution assay, were compared. The two assays were performed side by side using the same virus stock sample to determine the correlation between the results of the two assays. The TCID50 assay appeared to be more sensitive but slightly more variable, and there was a tenfold difference in the numerical results of these methods of enumeration. The advantages and disadvantages of both assays are discussed. Both methods are useful and practicable in filovirus research, and this comparison will be hugely beneficial to the filovirus research community as it seeks to become more united. Further work in this area should be performed to ensure consistency in filovirus research.
两种常见的量化丝状病毒感染力的方法，噬菌斑测定法和 50%细胞培养感染剂量（TCID50）终点稀释测定法，进行了比较。这两种测定法是使用相同的病毒库存样本同时进行的，以确定两种测定法结果之间的相关性。TCID50 测定法似乎更敏感，但略多变，这两种计数方法的数值结果相差十倍。讨论了这两种测定法的优缺点。这两种方法在丝状病毒研究中都很有用且切实可行，这种比较将极大地有益于丝状病毒研究界，因为它试图变得更加团结。应在该领域开展进一步的工作，以确保丝状病毒研究的一致性。
