TCID50/ml 和 FAID50/ml 法检测牛病毒滴度的比较。
Comparability of bovine virus titers obtained by TCID50/ml and FAID50/ml.
机构信息
Testing Facility for Biological Safety, Taiwan.
出版信息
J Virol Methods. 2010 Apr;165(1):121-4. doi: 10.1016/j.jviromet.2010.01.005. Epub 2010 Jan 28.
In this study, the correlation or comparability was assessed in the values of virus titers measured by either infectivity assay [reported as 50% tissue culture infectious doses (TCID(50)/ml)] or by immunofluorescence assay [reported as 50% fluorescent antibody infectious dose (FAID(50)/ml)]. The results demonstrate that bovine virus titers measured in infected bovine turbinate cells by these two methods are comparable. Clear demonstration of the comparability of these two methods provides a choice of a method for measuring the titer of bovine viruses.
在这项研究中，通过感染性测定（以 50%组织培养感染剂量（TCID(50)/ml）报告）或免疫荧光测定（以 50%荧光抗体感染剂量（FAID(50)/ml）报告）测量的病毒滴度的相关性或可比性进行了评估。结果表明，这两种方法测量感染牛鼻甲细胞中的牛病毒滴度是可比的。这两种方法的可比性的明确证明为测量牛病毒滴度提供了一种方法选择。
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