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观察到噻嗪酶活性部位的一个稳定卡宾。

Observation of a stable carbene at the active site of a thiamin enzyme.

机构信息

Albrecht-von-Haller Institute, Göttingen Center for Molecular Biosciences, Ernst-Caspari-Haus, Georg-August University Göttingen, Göttingen, Germany.

出版信息

Nat Chem Biol. 2013 Aug;9(8):488-90. doi: 10.1038/nchembio.1275. Epub 2013 Jun 9.

DOI:10.1038/nchembio.1275
PMID:23748673
Abstract

Carbenes are highly reactive chemical compounds that are exploited as ligands in organometallic chemistry and are powerful organic catalysts. They were postulated to occur as transient intermediates in enzymes, yet their existence in a biological system could never be demonstrated directly. We present spectroscopic and structural data of a thiamin enzyme in a noncovalent complex with substrate, which implicate accumulation of a stable carbene as a major resonance contributor to deprotonated thiamin.

摘要

碳烯是一种高反应性的化学化合物，在有机金属化学中被用作配体，也是一种强大的有机催化剂。它们被假定为酶中的瞬态中间体，但它们在生物系统中的存在从未被直接证明过。我们展示了一种硫胺素酶与底物非共价复合物的光谱和结构数据，这表明稳定碳烯的积累是去质子化硫胺素的主要共振贡献者。

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