使用三光子荧光寿命成像和Förster 共振能量转移显微镜研究活体细胞中的色氨酸-NADH 相互作用。
Investigation of tryptophan-NADH interactions in live human cells using three-photon fluorescence lifetime imaging and Förster resonance energy transfer microscopy.
出版信息
J Biomed Opt. 2013 Jun;18(6):060501. doi: 10.1117/1.JBO.18.6.060501.
Abstract
A method to investigate the metabolic activity of intracellular tryptophan (TRP) and coenzyme-NADH using three-photon (3P) fluorescence lifetime imaging (FLIM) and Förster resonance energy transfer (FRET) is presented. Through systematic analysis of FLIM data from tumorigenic and nontumorigenic cells, a statistically significant decrease in the fluorescence lifetime of TRP was observed in response to the increase in protein-bound NADH as cells were treated with glucose. The results demonstrate the potential use of 3P-FLIM-FRET as a tool for label-free screening of the change in metabolic flux occurring in human diseases or other clinical conditions.
摘要
介绍了一种使用三光子（3P）荧光寿命成像（FLIM）和Förster 共振能量转移（FRET）研究细胞内色氨酸（TRP）和辅酶-NADH 代谢活性的方法。通过对致瘤和非致瘤细胞的 FLIM 数据进行系统分析，发现随着细胞中葡萄糖处理后蛋白结合的 NADH 增加，TRP 的荧光寿命明显下降。结果表明，3P-FLIM-FRET 有可能成为一种无标记筛选工具，用于检测人类疾病或其他临床情况下发生的代谢通量变化。
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