• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Investigation of tryptophan-NADH interactions in live human cells using three-photon fluorescence lifetime imaging and Förster resonance energy transfer microscopy.使用三光子荧光寿命成像和Förster 共振能量转移显微镜研究活体细胞中的色氨酸-NADH 相互作用。
J Biomed Opt. 2013 Jun;18(6):060501. doi: 10.1117/1.JBO.18.6.060501.
2
Single-cell redox states analyzed by fluorescence lifetime metrics and tryptophan FRET interaction with NAD(P)H.利用荧光寿命指标分析单细胞氧化还原状态及色氨酸与 NAD(P)H 的 FRET 相互作用。
Cytometry A. 2019 Jan;95(1):110-121. doi: 10.1002/cyto.a.23711. Epub 2019 Jan 2.
3
Application of Fluorescence Lifetime Imaging (FLIM) to Measure Intracellular Environments in a Single Cell.应用荧光寿命成像（FLIM）测量单细胞内环境。
Adv Exp Med Biol. 2017;1035:121-133. doi: 10.1007/978-3-319-67358-5_8.
4
Dynamic FRET-FLIM based screening of signal transduction pathways.基于动态 FRET-FLIM 的信号转导通路筛选。
Sci Rep. 2021 Oct 20;11(1):20711. doi: 10.1038/s41598-021-00098-9.
5
A feasible add-on upgrade on a commercial two-photon FLIM microscope for optimal FLIM-FRET imaging of CFP-YFP pairs.在商业双光子 FLIM 显微镜上进行可行的附加升级，以实现 CFP-YFP 对的最佳 FLIM-FRET 成像。
J Fluoresc. 2013 May;23(3):543-9. doi: 10.1007/s10895-013-1188-8. Epub 2013 Mar 3.
6
Investigation of Mitochondrial Metabolic Response to Doxorubicin in Prostate Cancer Cells: An NADH, FAD and Tryptophan FLIM Assay.探讨前列腺癌细胞中阿霉素诱导的线粒体代谢反应：一种 NADH、FAD 和色氨酸 FLIM 检测方法。
Sci Rep. 2017 Sep 5;7(1):10451. doi: 10.1038/s41598-017-10856-3.
7
Quantitative FRET analysis by fast acquisition time domain FLIM at high spatial resolution in living cells.通过快速采集时域荧光寿命成像显微镜在活细胞中以高空间分辨率进行定量荧光共振能量转移分析。
Biophys J. 2008 Sep 15;95(6):2976-88. doi: 10.1529/biophysj.108.131276. Epub 2008 Jun 6.
8
Adaptive optics for a time-resolved Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) in vivo.用于体内时间分辨Förster 共振能量转移 (FRET) 和荧光寿命成像显微镜 (FLIM) 的自适应光学。
Opt Lett. 2020 May 15;45(10):2732-2735. doi: 10.1364/OL.385950.
9
The use of FLIM-FRET for the detection of mitochondria-associated protein interactions.使用荧光寿命成像-荧光共振能量转移技术检测线粒体相关蛋白相互作用。
Methods Mol Biol. 2015;1264:395-419. doi: 10.1007/978-1-4939-2257-4_34.
10
Quantitative real-time imaging of intracellular FRET biosensor dynamics using rapid multi-beam confocal FLIM.利用快速多光束共焦 FLIM 对细胞内 FRET 生物传感器动态进行定量实时成像。
Sci Rep. 2020 Mar 20;10(1):5146. doi: 10.1038/s41598-020-61478-1.

引用本文的文献

1
FLIMB: fluorescence lifetime microendoscopy for metabolic and functional imaging of femoral marrow at subcellular resolution.FLIMB：用于亚细胞分辨率下股骨骨髓代谢和功能成像的荧光寿命显微内镜检查。
Biomed Opt Express. 2025 Mar 31;16(4):1711-1731. doi: 10.1364/BOE.549311. eCollection 2025 Apr 1.
2
Structure, Function, and Thermodynamics of Lactate Dehydrogenases from Humans and the Malaria Parasite .人源和疟原虫乳酸脱氢酶的结构、功能和热力学性质
Biochemistry. 2021 Nov 30;60(47):3582-3595. doi: 10.1021/acs.biochem.1c00470. Epub 2021 Nov 8.
3
Special Section Guest Editorial: Thirty Years of Multiphoton Microscopy in the Biomedical Sciences.特刊社论：多光子显微镜在生物医学科学领域三十年的发展。
J Biomed Opt. 2020 Jan;25(1):1-3. doi: 10.1117/1.JBO.25.1.014501.
4
Recent trends in two-photon auto-fluorescence lifetime imaging (2P-FLIM) and its biomedical applications.双光子自发荧光寿命成像（2P-FLIM）的最新趋势及其生物医学应用。
Biomed Eng Lett. 2019 Jul 1;9(3):293-310. doi: 10.1007/s13534-019-00119-7. eCollection 2019 Aug.
5
Segmented cell analyses to measure redox states of autofluorescent NAD(P)H, FAD & Trp in cancer cells by FLIM.通过 FLIM 对癌细胞中自体荧光 NAD(P)H、FAD 和色氨酸的氧化还原状态进行分段细胞分析。
Sci Rep. 2018 Jan 8;8(1):79. doi: 10.1038/s41598-017-18634-x.
6
Investigation of Mitochondrial Metabolic Response to Doxorubicin in Prostate Cancer Cells: An NADH, FAD and Tryptophan FLIM Assay.探讨前列腺癌细胞中阿霉素诱导的线粒体代谢反应：一种 NADH、FAD 和色氨酸 FLIM 检测方法。
Sci Rep. 2017 Sep 5;7(1):10451. doi: 10.1038/s41598-017-10856-3.
7
Detection of urinary bladder cancer cells using redox ratio and double excitation wavelengths autofluorescence.利用氧化还原比率和双激发波长自发荧光检测膀胱癌细胞
Biomed Opt Express. 2015 Feb 25;6(3):977-86. doi: 10.1364/BOE.6.000977. eCollection 2015 Mar 1.
8
Intrinsic tryptophan fluorescence in the detection and analysis of proteins: a focus on Förster resonance energy transfer techniques.蛋白质检测与分析中的内源性色氨酸荧光：聚焦于福斯特共振能量转移技术
Int J Mol Sci. 2014 Dec 5;15(12):22518-38. doi: 10.3390/ijms151222518.

本文引用的文献

1
Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy.使用荧光寿命成像显微镜研究活细胞中的蛋白质-蛋白质相互作用。
Nat Protoc. 2011 Aug 11;6(9):1324-40. doi: 10.1038/nprot.2011.364.
2
Two-photon autofluorescence dynamics imaging reveals sensitivity of intracellular NADH concentration and conformation to cell physiology at the single-cell level.双光子自发荧光动力学成像揭示了单细胞水平下细胞内烟酰胺腺嘌呤二核苷酸（NADH）浓度和构象对细胞生理状态的敏感性。
J Photochem Photobiol B. 2009 Apr 2;95(1):46-57. doi: 10.1016/j.jphotobiol.2008.12.010. Epub 2008 Dec 25.
3
In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia.正常上皮和癌前上皮中蛋白质结合型和游离烟酰胺腺嘌呤二核苷酸的体内多光子荧光寿命成像
J Biomed Opt. 2007 Mar-Apr;12(2):024014. doi: 10.1117/1.2717503.
4
Energy metabolism in tumor cells.肿瘤细胞中的能量代谢。
FEBS J. 2007 Mar;274(6):1393-418. doi: 10.1111/j.1742-4658.2007.05686.x.
5
Metabolic mapping of MCF10A human breast cells via multiphoton fluorescence lifetime imaging of the coenzyme NADH.通过辅酶NADH的多光子荧光寿命成像对MCF10A人乳腺细胞进行代谢图谱分析。
Cancer Res. 2005 Oct 1;65(19):8766-73. doi: 10.1158/0008-5472.CAN-04-3922.
6
Characterization of two-photon excitation fluorescence lifetime imaging microscopy for protein localization.用于蛋白质定位的双光子激发荧光寿命成像显微镜的特性研究
Microsc Res Tech. 2004 Jan 1;63(1):72-80. doi: 10.1002/jemt.10430.
7
Characterization of the autofluorescence of polymorphonuclear leukocytes, mononuclear leukocytes and cervical epithelial cancer cells for improved spectroscopic discrimination of inflammation from dysplasia.
Photochem Photobiol. 2000 Mar;71(3):327-32.
8
Measuring serotonin distribution in live cells with three-photon excitation.利用三光子激发测量活细胞中血清素的分布。
Science. 1997 Jan 24;275(5299):530-2. doi: 10.1126/science.275.5299.530.
9
Lifetimes and NADH quenching of tryptophan fluorescence in pig heart lactate dehydrogenase.猪心脏乳酸脱氢酶中色氨酸荧光的寿命及NADH猝灭
Biochemistry. 1979 Jan 23;18(2):385-90. doi: 10.1021/bi00569a024.

使用三光子荧光寿命成像和Förster 共振能量转移显微镜研究活体细胞中的色氨酸-NADH 相互作用。

Investigation of tryptophan-NADH interactions in live human cells using three-photon fluorescence lifetime imaging and Förster resonance energy transfer microscopy.

出版信息

J Biomed Opt. 2013 Jun;18(6):060501. doi: 10.1117/1.JBO.18.6.060501.

DOI:10.1117/1.JBO.18.6.060501
PMID:23748699
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3675329/
Abstract

A method to investigate the metabolic activity of intracellular tryptophan (TRP) and coenzyme-NADH using three-photon (3P) fluorescence lifetime imaging (FLIM) and Förster resonance energy transfer (FRET) is presented. Through systematic analysis of FLIM data from tumorigenic and nontumorigenic cells, a statistically significant decrease in the fluorescence lifetime of TRP was observed in response to the increase in protein-bound NADH as cells were treated with glucose. The results demonstrate the potential use of 3P-FLIM-FRET as a tool for label-free screening of the change in metabolic flux occurring in human diseases or other clinical conditions.

摘要

介绍了一种使用三光子（3P）荧光寿命成像（FLIM）和Förster 共振能量转移（FRET）研究细胞内色氨酸（TRP）和辅酶-NADH 代谢活性的方法。通过对致瘤和非致瘤细胞的 FLIM 数据进行系统分析，发现随着细胞中葡萄糖处理后蛋白结合的 NADH 增加，TRP 的荧光寿命明显下降。结果表明，3P-FLIM-FRET 有可能成为一种无标记筛选工具，用于检测人类疾病或其他临床情况下发生的代谢通量变化。