Nakabayashi Takakazu, Awasthi Kamlesh, Ohta Nobuhiro
Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.
Department of Applied Chemistry and Institute of Molecular Science, National Chiao Tung University, 1001, Ta-Hsueh Road, Hsinchu, 30010, Taiwan.
Adv Exp Med Biol. 2017;1035:121-133. doi: 10.1007/978-3-319-67358-5_8.
Fluorescence lifetime imaging (FLIM) has now been used in many bioscience fields, which comes from the quantification of fluorescence lifetime. The procedure for obtaining lifetime images is very similar to that used in fluorescence microscopy. However, obtaining reliable lifetime images requires an understanding of the theory of fluorescence lifetime, principle of FLIM systems, and evaluation procedure of intracellular environments. In this chapter, the materials, methods, and notes on FLIM measurements have been described, in conjunction with a brief explanation of the background of FLIM.
荧光寿命成像（FLIM）现已应用于许多生物科学领域，它源于对荧光寿命的量化。获取寿命图像的过程与荧光显微镜中使用的过程非常相似。然而，要获得可靠的寿命图像，需要了解荧光寿命理论、FLIM系统原理以及细胞内环境的评估程序。在本章中，已描述了FLIM测量的材料、方法和注意事项，并对FLIM的背景进行了简要解释。
