Favazza Tara L, Tanimoto Naoyuki, Munro Robert J, Beck Susanne C, Garcia Garrido Marina, Seide Christina, Sothilingam Vithiyanjali, Hansen Ronald M, Fulton Anne B, Seeliger Mathias W, Akula James D
Department of Ophthalmology, Boston Children's Hospital, 300 Longwood Avenue, Fegan 4, Boston, MA, 02115, USA.
Doc Ophthalmol. 2013 Aug;127(1):3-11. doi: 10.1007/s10633-013-9392-z. Epub 2013 Jun 8.
To study the relationship between retinal and tunica vasculosa lentis (TVL) disease in retinopathy of prematurity (ROP). Although the clinical hallmark of ROP is abnormal retinal blood vessels, the vessels of the anterior segment, including the TVL, are also altered.
ROP was induced in Long-Evans pigmented and Sprague Dawley albino rats; room-air-reared (RAR) rats served as controls. Then, fluorescein angiographic images of the TVL and retinal vessels were serially obtained with a scanning laser ophthalmoscope near the height of retinal vascular disease, ~20 days of age, and again at 30 and 64 days of age. Additionally, electroretinograms (ERGs) were obtained prior to the first imaging session. The TVL images were analyzed for percent coverage of the posterior lens. The tortuosity of the retinal arterioles was determined using Retinal Image multiScale Analysis (Gelman et al. in Invest Ophthalmol Vis Sci 46:4734-4738, 2005).
In the youngest ROP rats, the TVL was dense, while in RAR rats, it was relatively sparse. By 30 days, the TVL in RAR rats had almost fully regressed, while in ROP rats, it was still pronounced. By the final test age, the TVL had completely regressed in both ROP and RAR rats. In parallel, the tortuous retinal arterioles in ROP rats resolved with increasing age. ERG components indicating postreceptoral dysfunction, the b-wave, and oscillatory potentials were attenuated in ROP rats.
These findings underscore the retinal vascular abnormalities and, for the first time, show abnormal anterior segment vasculature in the rat model of ROP. There is delayed regression of the TVL in the rat model of ROP. This demonstrates that ROP is a disease of the whole eye.
研究早产儿视网膜病变（ROP）中视网膜与晶状体血管膜（TVL）疾病之间的关系。尽管ROP的临床特征是视网膜血管异常，但前段血管，包括TVL，也会发生改变。
在长-伊文斯有色大鼠和斯普拉格-道利白化大鼠中诱导ROP；在空气中饲养（RAR）的大鼠作为对照。然后，在视网膜血管疾病高峰期（约20日龄）附近，用扫描激光检眼镜连续获取TVL和视网膜血管的荧光血管造影图像，并在30日龄和64日龄时再次获取。此外，在第一次成像前记录视网膜电图（ERG）。分析TVL图像以确定晶状体后表面的覆盖百分比。使用视网膜图像多尺度分析（Gelman等人，《Invest Ophthalmol Vis Sci》46:4734 - 4738，2005）确定视网膜小动脉的迂曲度。
在最年幼的ROP大鼠中，TVL密集，而在RAR大鼠中相对稀疏。到30日龄时，RAR大鼠的TVL几乎完全消退，而ROP大鼠的TVL仍然明显。到最终测试年龄时，ROP和RAR大鼠的TVL均已完全消退。与此同时，ROP大鼠中迂曲的视网膜小动脉随着年龄增长而恢复正常。ROP大鼠中指示感受器后功能障碍的ERG成分、b波和振荡电位均减弱。
这些发现强调了视网膜血管异常，并首次在ROP大鼠模型中显示前段血管系统异常。ROP大鼠模型中TVL的消退延迟。这表明ROP是一种全眼病。
