Institute of Bioinformatics, University of Georgia, GA 30605, USA.
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W454-8. doi: 10.1093/nar/gkt472. Epub 2013 Jun 8.
N-linked glycosylation is a posttranslational modification that has significantly contributed to the rapid evolution of HIV-1. In particular, enrichment of N-linked glycosylation sites can be found within Envelope variable loops, regions that play an essential role in HIV pathogenesis and immunogenicity. The web server described here, the HIV N-linked Glycosylation Site Analyzer, was developed to facilitate study of HIV diversity by tracking gp120 N-linked glycosylation sites. This server provides an automated platform for mapping and comparing variable loop N-linked glycosylation sites across populations of HIV-1 sequences. Furthermore, this server allows for refinement of HIV-1 sequence alignment by using N-linked glycosylation sites in variable loops as alignment anchors. Availability of this web server solves one of the difficult problems in HIV gp120 alignment and analysis imposed by the extraordinary HIV-1 diversity. The HIV N-linked Glycosylation Site Analyzer web server is available at http://hivtools.publichealth.uga.edu/N-Glyco/.
N-连接糖基化是一种翻译后修饰，它极大地促进了 HIV-1 的快速进化。特别是，在包膜的可变环中可以发现 N-连接糖基化位点的富集，这些区域在 HIV 的发病机制和免疫原性中起着至关重要的作用。这里描述的网络服务器，即 HIV N-连接糖基化位点分析器，是为了通过跟踪 gp120 N-连接糖基化位点来促进 HIV 多样性的研究而开发的。该服务器为映射和比较 HIV-1 序列群体中的可变环 N-连接糖基化位点提供了一个自动化的平台。此外，该服务器允许使用可变环中的 N-连接糖基化位点作为对齐锚点来细化 HIV-1 序列对齐。该网络服务器的可用性解决了由 HIV gp120 对齐和分析中 HIV-1 多样性带来的一个难题。HIV N-连接糖基化位点分析器网络服务器可在 http://hivtools.publichealth.uga.edu/N-Glyco/ 上获得。
