Lab of Modern Biotechnology, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, 230036, China.
Plant Cell Rep. 2013 Oct;32(10):1543-55. doi: 10.1007/s00299-013-1466-6. Epub 2013 Jun 9.
In this study, we identified 34 CCCH Znf genes in Medicago truncatula and the results of semi-quantitative RT-PCR revealed that the expression patterns of subfamily VI members were diverse. CCCH-type zinc finger (Znf) proteins are specific transcriptional factors with a typical motif consisting of three cysteine residues and one histidine residue. Increasing evidences have revealed that CCCH Znf proteins participated in the regulation of plant growth, developmental processes and environmental responses. Survey and characterization of CCCH Znf genes in leguminous species would facilitate a better understanding of the evolutionary processes and functions of this gene family. In this study, we performed a comprehensive analysis of CCCH Znf genes in M. truncatula by describing the phylogenetic relationships, chromosomal location and gene structure of each family member. A total of 34 CCCH Znf genes were identified in the latest M. truncatula genome sequence. The 34 predicted members were clustered into nine subfamilies based on their phylogenetic analysis and structure features. In addition, the 34 Medicago CCCH Znf genes were found to be unevenly distributed on eight chromosomes. Furthermore, the expression profiles of subfamily VI were investigated under different stress conditions (PEG-6000, NaCl and ABA) by using semi-quantitative RT-PCR. The data showed that these genes displayed different expression levels in response to various stress conditions. The results presented in this study provide basic information about Medicago CCCH Znf genes and form a fundamental clue for cloning genes with specific functions in further studies and applications.
在这项研究中，我们在蒺藜苜蓿中鉴定了 34 个 CCCH Znf 基因，半定量 RT-PCR 的结果表明，亚家族 VI 成员的表达模式具有多样性。CCCH 型锌指（Znf）蛋白是具有典型基序的特异性转录因子，该基序由三个半胱氨酸残基和一个组氨酸残基组成。越来越多的证据表明，CCCH Znf 蛋白参与了植物生长、发育过程和环境响应的调节。对豆科植物 CCCH Znf 基因的调查和特征分析将有助于更好地理解该基因家族的进化过程和功能。在这项研究中，我们通过描述每个家族成员的系统发育关系、染色体定位和基因结构，对蒺藜苜蓿中的 CCCH Znf 基因进行了全面分析。在最新的蒺藜苜蓿基因组序列中，共鉴定出 34 个 CCCH Znf 基因。根据系统发育分析和结构特征，这 34 个预测成员被聚类为九个亚家族。此外，在 34 个 Medicago CCCH Znf 基因中发现不均匀地分布在八个染色体上。此外，通过半定量 RT-PCR 研究了亚家族 VI 在不同胁迫条件（PEG-6000、NaCl 和 ABA）下的表达谱。结果表明，这些基因在响应各种胁迫条件时表现出不同的表达水平。本研究提供了蒺藜苜蓿 CCCH Znf 基因的基本信息，并为进一步研究和应用中克隆具有特定功能的基因提供了基础线索。
