College of Bioengineering, Henan University of Technology, Zhengzhou 450001, Henan Province, China.
Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan Province, China.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4108-4112. doi: 10.1099/ijs.0.049460-0. Epub 2013 Jun 7.
A novel actinobacterial strain, 3-wff-81(T), was isolated from interfacial sediment of the eutrophic Taihu Lake in Jiangsu Province (China) and subjected to polyphasic taxonomic characterization. The strain formed pale orange-pigmented colonies comprising rod-shaped cells on R2A agar. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain 3-wff-81(T) belonged to the genus Geodermatophilus, with Geodermatophilus soli PB34(T) (99.1 % similarity) and Geodermatophilus terrae PB261(T) (98.3 % similarity) as closest relatives. The major fatty acids were 16 : 0 iso, 15 : 0 iso, 17 : 1ω8c and 14 : 0 iso. The predominant menaquinones were MK-9(H4) and MK-9. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The genomic DNA G+C content was 73.2 mol%. DNA-DNA relatedness values with G. soli PB34(T) and G. terrae PB261(T) were 42.8 % and 39.6 %, respectively. Based on the physiological, biochemical and chemotaxonomic data, it is proposed that strain 3-wff-81(T) represents a novel species named Geodermatophilus taihuensis sp. nov. with 3-wff-81(T) ( = CGMCC 1.12303(T) = NBRC 109416(T)) as the type strain.
一株新的放线菌菌株 3-wff-81(T) 从江苏省太湖富营养化界面沉积物中分离出来，并进行了多相分类鉴定。该菌株在 R2A 琼脂上形成淡橙色色素的菌落，由杆状细胞组成。基于 16S rRNA 基因序列的系统发育分析表明，菌株 3-wff-81(T) 属于地衣芽孢杆菌属，与地衣芽孢杆菌 PB34(T)(99.1%相似性)和地衣芽孢杆菌 PB261(T)(98.3%相似性)亲缘关系最近。主要脂肪酸为 16:0 iso、15:0 iso、17:1ω8c 和 14:0 iso。主要的类异戊二烯醌为 MK-9(H4)和 MK-9。极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。基因组 DNA G+C 含量为 73.2mol%。与 G. soli PB34(T)和 G. terrae PB261(T)的 DNA-DNA 相关度分别为 42.8%和 39.6%。基于生理、生化和化学分类学数据，建议菌株 3-wff-81(T)代表一个新的种，命名为太湖地衣芽孢杆菌 sp. nov.，其 3-wff-81(T)(=CGMCC 1.12303(T)=NBRC 109416(T))为模式菌株。
