Laboratory of Soil Ecology, ABACUS, Cinvestav, Mexico.
Unidad de Microscopia Electrónica, Cinvestav, Mexico.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4163-4166. doi: 10.1099/ijs.0.053629-0. Epub 2013 Jun 7.
A novel haloalkaliphilic archaeon, strain B23(T) was isolated from the former lake Texcoco in Mexico. The strain was Gram-stain-negative, the cells coccoid to ovoid rods, red pigmented and aerobic. Strain B23(T) grew in 1.7-4.3 M NaCl, at pH 6.5-9.5 and at 25-45 °C with optimal growth at 2.6-3.4 M NaCl, pH 7.5-8.5 and 37 °C. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain B23(T) was most closely related to Natronobacterium gregoryi SP2(T) with 97.3 % sequence similarity. The polar lipids of strain B23(T) were phosphatidylglycerol and several unidentified phospholipids. The G+C content of the DNA of the strain was 62.5 mol%. Levels of DNA-DNA relatedness between strain B23(T) and Natronobacterium gregoryi DSM 3393(T) was 32.3 %. The name Natronobacterium texcoconense sp. nov. is proposed. The type strain is B23(T) ( = CECT 8068(T) = JCM 17655(T)).
一株新型嗜盐嗜碱古菌，菌株 B23(T)，从墨西哥前特斯科科湖分离得到。该菌株革兰氏阴性，细胞呈球形到卵形杆状，红色素，好氧。菌株 B23(T)在 1.7-4.3 M NaCl、pH 6.5-9.5 和 25-45°C 下生长，最佳生长条件为 2.6-3.4 M NaCl、pH 7.5-8.5 和 37°C。基于 16S rRNA 基因序列的系统发育分析表明，菌株 B23(T)与 Natronobacterium gregoryi SP2(T)的亲缘关系最密切，相似度为 97.3%。菌株 B23(T)的极性脂为磷脂酰甘油和几种未鉴定的磷脂。该菌株的 DNA G+C 含量为 62.5 mol%。菌株 B23(T)与 Natronobacterium gregoryi DSM 3393(T)之间的 DNA-DNA 同源性水平为 32.3%。建议将该菌株命名为 Natronobacterium texcoconense sp. nov.。其模式菌株为 B23(T)（=CECT 8068(T)=JCM 17655(T)）。
