Laboratory of Soil Ecology, Cinvestav, Mexico City, Mexico.
Arch Microbiol. 2013 Feb;195(2):145-51. doi: 10.1007/s00203-012-0852-8. Epub 2012 Nov 28.
A new haloalkaliphilic archaeon, strain B4(T), was isolated from the former lake Texcoco in Mexico. The cells were Gram-negative, pleomorphic-shaped, pink to red pigmented and aerobic. Strain B4(T) required at least 2.5 M NaCl for growth, with optimum growth at 3.4 M NaCl. It was able to grow over a pH range of 7.5-10.0 and temperature of 25-50 °C, with optimal growth at pH 9 and 37 °C. Cells are lysed in hypotonic treatment with less than 1.3 M NaCl. The major polar lipids of strain B4(T) were phosphatidylglycerol and methyl-phosphatidylglycerophosphate. Phospholipids were detected, but not glycolipids. The nucleotide sequence of the 16S rRNA gene revealed that the strain B4(T) was phylogenetically related to members of the genus Natronorubrum. Sequence similarity with Natronorubrum tibetense was 96.28 %, with Natronorubrum sulfidifaciens 95.06 % and Natronorubrum sediminis 94.98 %. The G+C content of the DNA was 63.3 mol%. The name of Natronorubrum texcoconense sp. nov. is proposed. The type strain is B4(T) (=CECT 8067(T) = JCM 17497(T)).
一种新的嗜盐嗜碱古菌，菌株 B4(T)，从墨西哥前特斯科科湖(Texcoco Lake)中分离得到。细胞呈革兰氏阴性、多形性、粉红色到红色色素、需氧。菌株 B4(T)生长至少需要 2.5 M NaCl，最适生长在 3.4 M NaCl 中。它能够在 pH 值为 7.5-10.0 和温度为 25-50°C 的范围内生长，最适生长在 pH 值为 9 和 37°C。细胞在低于 1.3 M NaCl 的低渗处理下被裂解。菌株 B4(T)的主要极性脂为磷脂酰甘油和甲基磷脂酰甘油磷酸酯。检测到磷脂，但未检测到糖脂。16S rRNA 基因的序列表明，菌株 B4(T)与 Natronorubrum 属的成员在系统发育上相关。与 Natronorubrum tibetense 的序列相似性为 96.28%，与 Natronorubrum sulfidifaciens 为 95.06%，与 Natronorubrum sediminis 为 94.98%。DNA 的 G+C 含量为 63.3 mol%。建议使用 Natronorubrum texcoconense sp. nov. 这个名称。模式菌株为 B4(T)(=CECT 8067(T)=JCM 17497(T))。
