Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
Soil Fertilizer and Environment Energy Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, PR China.
Int J Syst Evol Microbiol. 2010 Oct;60(Pt 10):2267-2271. doi: 10.1099/ijs.0.013995-0. Epub 2009 Nov 13.
A haloalkaliphilic archaeon, strain JX313(T), was isolated from a saline-alkaline soil from Daqing, Heilongjiang Province, China. Its morphological, physiological and biochemical features and 16S rRNA gene sequence were determined. Colonies of the strain were orange-red and cells were non-motile cocci and Gram-stain-variable. The strain required at least 1.7 M NaCl for growth, with optimal growth occurring in 2.0-2.5 M NaCl. Growth was observed at 20-50°C and pH 8.0-10.5, with optimal growth at 35°C and pH 10.0. The G+C content of its genomic DNA was 59.3 mol%. Phylogenetic analysis of 16S rRNA gene sequences showed that strain JX313(T) is associated with the genera Haloterrigena and Natrinema and is most closely related to Haloterrigena salina XH-65(T) (96.2 % sequence similarity) and Haloterrigena hispanica FP1(T) (96.2 %). DNA-DNA hybridization experiments revealed that the relatedness of strain JX313(T) to type strains of related species of the genus Haloterrigena or Natrinema was less than 50 %. Furthermore, the cellular polar lipids of strain JX313(T), identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and mannose-2,6-disulfate (1→2)-glucose glycerol diether (S₂-DGD), were consistent with the polar lipid characteristics of the genus Haloterrigena. Therefore, phylogenetic analysis, phenotypic assessment and chemotaxonomic data showed that JX313(T) represents a novel species within the genus Haloterrigena, for which the name Haloterrigena daqingensis sp. nov. is proposed. The type strain is JX313(T) (=CGMCC 1.8909(T) =NBRC 105739(T)).
一株嗜盐-耐碱古菌，菌株 JX313(T)，从中国黑龙江大庆的盐碱土壤中分离得到。其形态、生理生化特征和 16S rRNA 基因序列已确定。该菌株的菌落呈橙红色，细胞为非运动性球菌，革兰氏染色可变。该菌株的生长至少需要 1.7 M NaCl，在 2.0-2.5 M NaCl 中生长最佳。在 20-50°C 和 pH 8.0-10.5 下观察到生长，最适生长温度为 35°C，最适 pH 值为 10.0。其基因组 DNA 的 G+C 含量为 59.3 mol%。16S rRNA 基因序列的系统发育分析表明，菌株 JX313(T)与 Haloterrigena 和 Natrinema 属有关，与 Haloterrigena salina XH-65(T)（96.2%序列相似性）和 Haloterrigena hispanica FP1(T)（96.2%序列相似性）最为密切相关。DNA-DNA 杂交实验表明，菌株 JX313(T)与 Haloterrigena 属或 Natrinema 属相关种的模式菌株的相关性小于 50%。此外，菌株 JX313(T)的细胞极性脂鉴定为磷脂酰甘油、磷脂酰甘油磷酸甲基酯和甘露糖-2,6-二硫酸盐（1→2）-葡萄糖甘油二醚（S₂-DGD），与 Haloterrigena 属的极性脂特征一致。因此，系统发育分析、表型评估和化学分类数据表明，JX313(T)代表 Haloterrigena 属的一个新种，命名为 Haloterrigena daqingensis sp. nov.。模式菌株为 JX313(T)（=CGMCC 1.8909(T) =NBRC 105739(T)）。
