Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Institute for Microelectronics and Microsystems, National Research Council (IMM-CNR) and Sens&Micro LAB, 73100 Lecce, Italy.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):104-108. doi: 10.1099/ijs.0.036558-0. Epub 2012 Feb 17.
A novel halophilic archaeon, strain CG-1(T), belonging to the genus Natronococcus was isolated from sediment of the soda lake Chagannor in Inner Mongolia, China. The colonies of this strain were pink pigmented, the intensity of the colour decreased when the cells grew at salt saturation levels. The cells were non-motile cocci and strictly aerobic. Hypotonic treatment did not cause cell lysis, even in distilled water. Strain CG-1(T) grew at 15-30.0 % (w/v) NaCl and at 30-50 °C and pH 8.0-11.0, with optimal growth occurring at 25-30 % (w/v) NaCl, 37-45 °C and pH 9-9.5. MgCl(2) was not required for growth. Strain CG-1(T) was most closely related to the type strains of Natronococcus amylolyticus Ah-36(T), Natronococcus jeotgali B1(T) and Natronococcus occultus SP4(T), with which it shared 98.4 %, 96.2 and 95.7 % 16S rRNA gene sequence similarity, respectively. The polar lipids consisted of C(20)C(20) and C(20)C(25) derivatives of phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me) and minor phospholipid components. No glycolipids were detected. The DNA G+C content of strain CG-1(T) was 62.1 mol%. DNA-DNA hybridization with N. amylolyticus DSM 10524(T), phylogenetically the most closely related species, was 39 %; this value showed that strain CG-1(T) constituted a different genospecies. The comparison of 16S rRNA gene sequences, detailed phenotypic characterization, polar lipid profile and DNA-DNA hybridization studies revealed that strain CG-1(T) belongs to the genus Natronococcus and constitutes a novel species for which the name Natronococcus roseus sp. nov. is proposed. The type strain is CG-1(T) (=CECT 7984(T)=IBRC-M 10656(T)=JCM 17958(T)).
一株新的嗜盐古菌，菌株 CG-1(T)，属于嗜盐球菌属，从中国内蒙古苏打湖 Chagannor 的沉积物中分离得到。该菌株的菌落呈粉红色，当细胞在盐饱和水平下生长时，颜色强度会降低。细胞是非运动性球菌，严格需氧。低渗处理不会导致细胞裂解，即使在蒸馏水中也是如此。菌株 CG-1(T)在 15-30.0%（w/v）NaCl 和 30-50°C 及 pH8.0-11.0 条件下生长，最佳生长条件为 25-30%（w/v）NaCl、37-45°C 和 pH9-9.5。生长不需要 MgCl2。菌株 CG-1(T)与嗜盐球菌属的模式菌株纳他球菌淀粉液化亚种 Ah-36(T)、纳他球菌杰氏亚种 B1(T)和纳他球菌隐种 SP4(T)最为密切相关，它们的 16S rRNA 基因序列相似性分别为 98.4%、96.2%和 95.7%。极性脂由磷脂酰甘油（PG）、磷酸甘油磷酸甲酯（PGP-Me）和少量磷脂成分的 C20C20 和 C20C25 衍生物组成。未检测到糖脂。菌株 CG-1(T)的 DNA G+C 含量为 62.1mol%。与亲缘关系最密切的种纳他球菌淀粉液化亚种 DSM 10524(T)的 DNA-DNA 杂交率为 39%；这一数值表明，菌株 CG-1(T)构成了一个不同的种。16S rRNA 基因序列比较、详细表型特征、极性脂谱和 DNA-DNA 杂交研究表明，菌株 CG-1(T)属于嗜盐球菌属，是一个新的种，建议命名为玫瑰嗜盐球菌（Natronococcus roseus）。模式菌株为 CG-1(T)=CECT 7984(T)=IBRC-M 10656(T)=JCM 17958(T)。
