Lu Xiong-Xiong, Zhang Shu-Min, Fang Yuan, Wang Zheng-Ting, Xie Jun-Jie, Zhan Qian, Deng Xia-Xing, Chen Hao, Jin Jia-Bin, Peng Cheng-Hong, Li Hong-Wei, Shen Bai-Yong
Center of Organ Transplantation, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, No. 197, Ruijiner Road, Shanghai, 200025, China.
Tumour Biol. 2013 Dec;34(6):3339-43. doi: 10.1007/s13277-013-0903-z. Epub 2013 Jun 8.
The aim of this study was to analyze the clinical significance of reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) promoter methylation in pancreatic ductal adenocarcinoma (PDA). Methylation-specific polymerase chain reaction was used to examine the promoter methylation status of RECK in 60 pairs of PDA tissue samples and adjacent non-cancerous tissue samples. Statistical analyses were applied to test the associations between RECK promoter methylation status, clinicopathologic factors, and prognosis. The rate of RECK promoter methylation was significantly higher in PDA tissues than in adjacent non-cancerous tissues (P < 0.001). RECK methylation status was significantly associated with clinical stage (P = 0.017), histological differentiation (P = 0.046), and lymph node metastasis (P = 0.003), but was not associated with gender, age, and tumor location (all P > 0.05). Additionally, RECK promoter methylation is associated with malignant behavior and poor prognosis. In conclusion, determination of RECK promoter methylation status in tumor tissues may assist in the identification of patients who require aggressive postoperative intervention in order to improve prognosis.
本研究旨在分析含Kazal基序的逆转诱导富含半胱氨酸蛋白（RECK）启动子甲基化在胰腺导管腺癌（PDA）中的临床意义。采用甲基化特异性聚合酶链反应检测60对PDA组织样本和癌旁非癌组织样本中RECK的启动子甲基化状态。应用统计学分析来检验RECK启动子甲基化状态、临床病理因素和预后之间的关联。PDA组织中RECK启动子甲基化率显著高于癌旁非癌组织（P < 0.001）。RECK甲基化状态与临床分期（P = 0.017）、组织学分化（P = 0.046）和淋巴结转移（P = 0.003）显著相关，但与性别、年龄和肿瘤位置无关（所有P > 0.05）。此外，RECK启动子甲基化与恶性行为和不良预后相关。总之，检测肿瘤组织中RECK启动子甲基化状态可能有助于识别需要积极术后干预以改善预后的患者。
