单壁碳纳米管介导的小干扰RNA递送用于人神经母细胞瘤中胃泌素释放肽受体的沉默
Single-walled carbon nanotube-mediated small interfering RNA delivery for gastrin-releasing peptide receptor silencing in human neuroblastoma.
作者信息
Qiao Jingbo, Hong Tu, Guo Honglian, Xu Ya-Qiong, Chung Dai H
机构信息
Department of Pediatric Surgery, Vanderbilt University Medical center, Nashville, TN, USA.
出版信息
Methods Mol Biol. 2013;1026:137-47. doi: 10.1007/978-1-62703-468-5_11.
Abstract
Small interfering RNA (siRNA) has the potential to influence gene expression with a high degree of target gene specificity. However, the clinical application of siRNA therapeutics has proven to be less promising as evidenced by its poor intracellular uptake, instability in vivo, and nonspecific immune stimulations. Recently, we have demonstrated that single-walled carbon nanotube (SWNT)-mediated siRNA delivery can enhance the efficiency of siRNA-mediated gastrin-releasing peptide receptor (GRP-R) gene silencing by stabilizing siRNA while selectively targeting tumor tissues. Based on our recent findings, we introduce a novel technique to silence specific gene(s) in human neuroblastoma through SWNT-mediated siRNA delivery in vitro and in vivo.
摘要
小干扰RNA（siRNA）具有以高度的靶基因特异性影响基因表达的潜力。然而，siRNA疗法的临床应用已被证明前景不太乐观，其细胞内摄取不佳、体内不稳定以及非特异性免疫刺激就是明证。最近，我们已经证明，单壁碳纳米管（SWNT）介导的siRNA递送可以通过稳定siRNA同时选择性靶向肿瘤组织来提高siRNA介导的胃泌素释放肽受体（GRP-R）基因沉默的效率。基于我们最近的发现，我们引入了一种新技术，通过在体外和体内SWNT介导的siRNA递送使人类神经母细胞瘤中的特定基因沉默。
相似文献
1
Single-walled carbon nanotube-mediated small interfering RNA delivery for gastrin-releasing peptide receptor silencing in human neuroblastoma.单壁碳纳米管介导的小干扰RNA递送用于人神经母细胞瘤中胃泌素释放肽受体的沉默
Methods Mol Biol. 2013;1026:137-47. doi: 10.1007/978-1-62703-468-5_11.
2
DOTAP functionalizing single-walled carbon nanotubes as non-viral vectors for efficient intracellular siRNA delivery.DOTAP 功能化单壁碳纳米管作为非病毒载体，用于高效的细胞内 siRNA 递送。
Drug Deliv. 2016;23(3):840-8. doi: 10.3109/10717544.2014.919542. Epub 2015 Sep 4.
3
Delivery of telomerase reverse transcriptase small interfering RNA in complex with positively charged single-walled carbon nanotubes suppresses tumor growth.端粒酶逆转录酶小干扰RNA与带正电荷的单壁碳纳米管复合递送可抑制肿瘤生长。
Clin Cancer Res. 2006 Aug 15;12(16):4933-9. doi: 10.1158/1078-0432.CCR-05-2831.
4
Gastrin-releasing peptide receptor silencing suppresses the tumorigenesis and metastatic potential of neuroblastoma.胃泌素释放肽受体沉默可抑制神经母细胞瘤的肿瘤发生和转移潜能。
Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12891-6. doi: 10.1073/pnas.0711861105. Epub 2008 Aug 27.
5
Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for SiRNA Delivery.通过DSPE-PEG-PEI对单壁碳纳米管进行非共价功能化用于小干扰RNA递送
Methods Mol Biol. 2016;1364:151-63. doi: 10.1007/978-1-4939-3112-5_13.
6
Functionalization of carbon nanotubes via cleavable disulfide bonds for efficient intracellular delivery of siRNA and potent gene silencing.通过可裂解的二硫键对碳纳米管进行功能化修饰，以实现小干扰RNA的高效细胞内递送和有效的基因沉默。
J Am Chem Soc. 2005 Sep 14;127(36):12492-3. doi: 10.1021/ja053962k.
7
Silencing gastrin-releasing peptide receptor suppresses key regulators of aerobic glycolysis in neuroblastoma cells.沉默胃泌素释放肽受体可抑制神经母细胞瘤细胞中糖酵解的关键调节因子。
Pediatr Blood Cancer. 2015 Apr;62(4):581-6. doi: 10.1002/pbc.25348. Epub 2015 Jan 28.
8
RNAi mediated gene silencing of ITPA using a targeted nanocarrier: Apoptosis induction in SKBR3 cancer cells.使用靶向纳米载体通过RNA干扰介导ITPA基因沉默：SKBR3癌细胞中的细胞凋亡诱导
Clin Exp Pharmacol Physiol. 2017 Aug;44(8):888-894. doi: 10.1111/1440-1681.12776.
9
Single-walled carbon nanotubes functionalized with aptamer and piperazine-polyethylenimine derivative for targeted siRNA delivery into breast cancer cells.经适体功能化单壁碳纳米管和哌嗪-聚亚乙基亚胺衍生物用于靶向递送 siRNA 进入乳腺癌细胞。
Int J Pharm. 2015 May 15;485(1-2):50-60. doi: 10.1016/j.ijpharm.2015.02.031. Epub 2015 Feb 21.
10
Magneto-Fluorescent Carbon Nanotube-Mediated siRNA for Gastrin-Releasing Peptide Receptor Silencing in Neuroblastoma.磁荧光碳纳米管介导的小干扰RNA用于神经母细胞瘤中胃泌素释放肽受体的沉默
RSC Adv. 2013 Apr 14;3(14):4544-4551. doi: 10.1039/C3RA23023F.
引用本文的文献
1
Controlled Mechanical Motions of Microparticles in Optical Tweezers.光镊中微粒的受控机械运动
Micromachines (Basel). 2018 May 12;9(5):232. doi: 10.3390/mi9050232.
2
Bombesin related peptides/receptors and their promising therapeutic roles in cancer imaging, targeting and treatment.蛙皮素相关肽/受体及其在癌症成像、靶向和治疗中的潜在治疗作用。
Expert Opin Ther Targets. 2016 Sep;20(9):1055-73. doi: 10.1517/14728222.2016.1164694. Epub 2016 Mar 28.
3
Carbon nanotubes: properties, synthesis, purification, and medical applications.碳纳米管：性质、合成、纯化及医学应用。
Nanoscale Res Lett. 2014 Aug 13;9(1):393. doi: 10.1186/1556-276X-9-393. eCollection 2014.
本文引用的文献
1
Effect of Competitive Surface Functionalization on Dual-Modality Fluorescence and Magnetic Resonance Imaging of Single-Walled Carbon Nanotubes.竞争性表面功能化对单壁碳纳米管双模态荧光和磁共振成像的影响
J Phys Chem C Nanomater Interfaces. 2012 Aug 2;116(30):16319-16324. doi: 10.1021/jp305372z. Epub 2012 Jul 3.
2
Dual-modality photothermal optical coherence tomography and magnetic-resonance imaging of carbon nanotubes.碳纳米管的双模态光热光学相干断层扫描和磁共振成像。
Opt Lett. 2012 Mar 1;37(5):872-4. doi: 10.1364/OL.37.000872.
3
A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice.一种用于在小鼠体内近红外成像的明亮荧光碳纳米管的途径。
Nat Nanotechnol. 2009 Nov;4(11):773-80. doi: 10.1038/nnano.2009.294. Epub 2009 Oct 11.
4
Gastrin-releasing peptide receptor silencing suppresses the tumorigenesis and metastatic potential of neuroblastoma.胃泌素释放肽受体沉默可抑制神经母细胞瘤的肿瘤发生和转移潜能。
Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12891-6. doi: 10.1073/pnas.0711861105. Epub 2008 Aug 27.
5
Bombesin induces angiogenesis and neuroblastoma growth.蛙皮素可诱导血管生成和神经母细胞瘤生长。
Cancer Lett. 2007 Aug 18;253(2):273-81. doi: 10.1016/j.canlet.2007.02.007. Epub 2007 Mar 26.
6
siRNA delivery into human T cells and primary cells with carbon-nanotube transporters.利用碳纳米管转运体将小干扰RNA递送至人T细胞和原代细胞。
Angew Chem Int Ed Engl. 2007;46(12):2023-7. doi: 10.1002/anie.200604295.
7
Mammalian pharmacokinetics of carbon nanotubes using intrinsic near-infrared fluorescence.利用固有近红外荧光研究碳纳米管的哺乳动物药代动力学。
Proc Natl Acad Sci U S A. 2006 Dec 12;103(50):18882-6. doi: 10.1073/pnas.0609265103. Epub 2006 Nov 29.
8
Carbon nanotubes as intracellular transporters for proteins and DNA: an investigation of the uptake mechanism and pathway.碳纳米管作为蛋白质和DNA的细胞内转运体：摄取机制和途径的研究
Angew Chem Int Ed Engl. 2006 Jan 16;45(4):577-81. doi: 10.1002/anie.200503389.
9
Functionalization of carbon nanotubes via cleavable disulfide bonds for efficient intracellular delivery of siRNA and potent gene silencing.通过可裂解的二硫键对碳纳米管进行功能化修饰，以实现小干扰RNA的高效细胞内递送和有效的基因沉默。
J Am Chem Soc. 2005 Sep 14;127(36):12492-3. doi: 10.1021/ja053962k.
10
Gastrin-releasing peptide-induced down-regulation of tumor suppressor protein PTEN (phosphatase and tensin homolog deleted on chromosome ten) in neuroblastomas.胃泌素释放肽诱导神经母细胞瘤中肿瘤抑制蛋白PTEN（第10号染色体缺失的磷酸酶及张力蛋白同源物）下调。
Ann Surg. 2005 May;241(5):684-91; discussion 691-2. doi: 10.1097/01.sla.0000161173.47717.71.
Zotero 插件