宫颈癌中的独特 microRNA 表达谱。

Unique microRNA expression profiles in cervical cancer.

机构信息

Institute of Public Health, University of Pecs, Pecs, Hungary.

出版信息

Anticancer Res. 2013 Jun;33(6):2561-7.

Abstract

Cervical cancer is the second leading cause of death among female patients with cancer in the world. Our aim was to analyze cervical cancer cases, in the Southwestern Transdanubian Region of Hungary, with regard to human papillomavirus (HPV) genotype and histological and clinical grading. After HPV testing and genotyping, the expressions of eight different pre-microRNAs (miR-21, miR-27a, miR-34a, miR-146a, miR-155, miR-196a, miR-203, miR-221) in formalin-fixed paraffin-embedded (FFPE) primary human cervical cancer samples were evaluated with the help of the LightCycler 480 PCR System (Roche). Statistically significant overexpression of miR-21 (p=0.004), miR-27a (p=0.018), miR-34a (p<0.001), miR-155 (p=0.021), miR-196a (p=0.032), miR-203 (p=0.037) and miR-221 (p=0.017) were observed in squamous cell carcinoma, regardless of HPV status and clinical grading. Significant overexpression of miR-21 (p=0.004), miR-27a (p=0.02), miR-34a (p<0.001), miR-196a (p=0.027) and miR-221 (p=0.031) was characteristic of HPV-positive squamous cell carcinomas in contrast to adenocarcinomas of the same HPV status.

摘要

宫颈癌是全球女性癌症患者的第二大死亡原因。我们的目的是分析匈牙利西南部多瑙河区的宫颈癌病例，分析其与人类乳头瘤病毒（HPV）基因型以及组织学和临床分级的关系。HPV 检测和基因分型后，借助 LightCycler 480 PCR 系统（罗氏）评估了 8 种不同的前 microRNA（miR-21、miR-27a、miR-34a、miR-146a、miR-155、miR-196a、miR-203、miR-221）在福尔马林固定石蜡包埋（FFPE）原发性人宫颈癌样本中的表达。miR-21（p=0.004）、miR-27a（p=0.018）、miR-34a（p<0.001）、miR-155（p=0.021）、miR-196a（p=0.032）、miR-203（p=0.037）和 miR-221（p=0.017）在鳞状细胞癌中表达显著上调，与 HPV 状态和临床分级无关。miR-21（p=0.004）、miR-27a（p=0.02）、miR-34a（p<0.001）、miR-196a（p=0.027）和 miR-221（p=0.031）在 HPV 阳性鳞状细胞癌中表达显著上调，与 HPV 状态相同的腺癌形成对比。