妊娠相关的宫颈非编码 RNA 变化。
Pregnancy-associated changes in cervical noncoding RNA.
机构信息
Department of Obstetrics & Gynecology, Maternal Child Health Research Center, Center for Research on Reproduction & Women's Health, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.
Department of Obstetrics & Gynecology, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
出版信息
Epigenomics. 2020 Jun;12(12):1013-1025. doi: 10.2217/epi-2019-0231. Epub 2020 Aug 18.
To identify pregnancy-associated changes in cervical noncoding RNA (ncRNA), including miRNA and long noncoding RNA (lncRNA), and their potential effects on biologic processes. We enrolled 21 pregnant women with term deliveries (≥37 weeks' gestation) in a prospective cohort and collected cervical swabs before 28 weeks' gestation. We enrolled 21 nonpregnant controls. We analyzed miRNA, lncRNA and mRNA expression, applying a Bonferroni correction. Five miRNA and three lncRNA were significantly differentially (>twofold change) expressed. Putative miRNA targets are enriched in genes mediating organogenesis, glucocorticoid signaling, cell adhesion and ncRNA machinery. Differential cervical ncRNA expression occurs in the setting of pregnancy. Gene ontology classification reveals biological pathways through which miRNA may play a biologic role in normal pregnancy physiology.
为了鉴定与妊娠相关的宫颈非编码 RNA（ncRNA），包括 microRNA 和长非编码 RNA（lncRNA），及其对生物学过程的潜在影响。我们前瞻性招募了 21 名足月分娩（≥37 周妊娠）的孕妇，并在 28 周妊娠前采集宫颈拭子。我们还招募了 21 名未怀孕的对照。我们应用 Bonferroni 校正分析了 miRNA、lncRNA 和 mRNA 的表达。有 5 个 miRNA 和 3 个 lncRNA 的表达有显著差异（>两倍变化）。推测的 miRNA 靶基因在介导器官发生、糖皮质激素信号、细胞黏附和 ncRNA 机制的基因中富集。在妊娠状态下，宫颈 ncRNA 的表达出现差异。GO 分类揭示了 miRNA 可能在正常妊娠生理中发挥生物学作用的生物学途径。
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