aDepartment of Pathology, Faculty of Medicine of the Eduardo Mondlane University and Maputo Central Hospital, Maputo bDepartment of Pathology, Beira Central Hospital, Beira, Mozambique cIPATIMUP - Institute of Molecular Pathology and Immunology dDepartment of Pathology, Faculty of Medicine eDepartment of Clinical Epidemiology, Predictive Medicine and Public Health, Faculty of Medicine of the University of Porto fInstitute of Public Health of the University of Porto (ISPUP), Porto, Portugal gDepartment of Pathology, Free University Hospital, Amsterdam, The Netherlands.
Eur J Cancer Prev. 2013 Nov;22(6):566-8. doi: 10.1097/CEJ.0b013e328363005d.
The infection with human papillomavirus (HPV) has been described as a risk factor for squamous cell carcinoma of the conjunctiva (SCCC), although the evidence is conflicting. To assess the relation between HPV infection and intraepithelial neoplasia or SCCC, we evaluated archived material from biopsies of the conjunctiva performed at the Maputo Central Hospital (Mozambique) in patients with suspected eye cancer. The quality of DNA was assessed by PCR using β-globin-specific primers. A total of 22 consecutive biopsies (intraepithelial neoplasia, SCCC, and benign conditions) positive for β-globin were further tested for HPV infection by PCR using the general primers GP5+/GP6+ and CPI/CPII. In addition, PCR with type-specific primers HPV 16 and HPV 18 was performed. Nineteen biopsies corresponded to intraepithelial neoplasia (two low-grade and nine high-grade) or SCCC (n=8), from which 11 (57.9%) tested positive for HPV infection; nine were positive for CPI/CPII, including one case also positive for GP5+/GP6+ and HPV 18, and the remaining two tested positive only for HPV 16. HPV DNA was not detected in any of the three biopsies of benign conditions. These results suggest a stronger association between infection with cutaneous HPV and SCCC than for mucosal HPV. However, further research is required to clarify the relation between HPV and SCCC as well as to understand the potential of the HPV vaccine currently available for cervical cancer to prevent SCCC.
人乳头瘤病毒（HPV）感染已被描述为结膜鳞状细胞癌（SCCC）的危险因素，尽管证据相互矛盾。为了评估 HPV 感染与上皮内瘤变或 SCCC 之间的关系，我们评估了莫桑比克马普托中央医院（Mozambique）对疑似眼癌患者进行的结膜活检存档材料。通过使用β-珠蛋白特异性引物的 PCR 评估 DNA 质量。共有 22 例连续的结膜活检（上皮内瘤变、SCCC 和良性病变）β-珠蛋白阳性，进一步使用通用引物 GP5+/GP6+和 CPI/CPII 通过 PCR 检测 HPV 感染。此外，还使用 HPV 16 和 HPV 18 型特异性引物进行 PCR。19 例活检对应上皮内瘤变（2 例低级别和 9 例高级别）或 SCCC（n=8），其中 11 例（57.9%）检测到 HPV 感染呈阳性；9 例为 CPI/CPII 阳性，包括 1 例也为 GP5+/GP6+和 HPV 18 阳性，其余 2 例仅为 HPV 16 阳性。在 3 例良性病变的活检中均未检测到 HPV DNA。这些结果表明，皮肤 HPV 感染与 SCCC 的相关性强于黏膜 HPV。然而，需要进一步的研究来阐明 HPV 与 SCCC 之间的关系，并了解目前用于宫颈癌的 HPV 疫苗预防 SCCC 的潜力。
