Jiang Shu, Yang Jing, Qian Dawei, Guo Jianming, Shang Er-xin, Duan Jin-ao, Xu Jun
Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing, 210046, People's Republic of China,
Arch Pharm Res. 2014 Feb;37(2):204-13. doi: 10.1007/s12272-013-0172-9. Epub 2013 Jun 11.
Ultra performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF MS) technique combined with Metabolynx™ software was used for analysis of the metabolites of quercitrin by the isolated human intestinal bacteria from the human feces. Four metabolites of quercitrin were detected and tentatively identified based on the characteristics of their protonated ions. The metabolites were metabolized by four main metabolic pathways including hydroxylation, demethylation, deglycosylation and ring-cleavage. Quercitrin was metabolized to the hydroxyquercitrin and desmethylquercitrin by the majority of the isolated intestinal bacteria such as Bacteroides sp. 54, and was degraded to the deglycosylated product quercetin by rhamnosidase and further ring-cleavage metabolite 3,4-dihydroxybenzoic acid by the minority of the isolated bacteria such as Bacteroides sp. 45. The metabolic pathways and most of the metabolites of quercitrin were reported for the first time.
采用超高效液相色谱/四极杆-飞行时间质谱（UPLC/Q-TOF MS）技术结合Metabolynx™软件，对从人类粪便中分离出的人体肠道细菌代谢槲皮苷的产物进行分析。根据质子化离子的特征，检测并初步鉴定出了槲皮苷的4种代谢产物。这些代谢产物通过羟基化、去甲基化、去糖基化和环裂解4种主要代谢途径进行代谢。大多数分离出的肠道细菌（如拟杆菌属54）可将槲皮苷代谢为羟基槲皮苷和去甲基槲皮苷，少数分离出的细菌（如拟杆菌属45）可通过鼠李糖苷酶将其降解为去糖基化产物槲皮素，并进一步环裂解为代谢产物3,4-二羟基苯甲酸。槲皮苷的代谢途径和大多数代谢产物均为首次报道。
