Liu Yixi, Harinantenaina Liva, Brodie Peggy J, Slebodnick Carla, Callmander Martin W, Rakotondrajaona R, Rakotobe Etienne, Rasamison Vincent E, TenDyke Karen, Shen Yongchun, Kingston David G I
Department of Chemistry and the Virginia Tech Center for Drug Discovery, Virginia Tech, Blacksburg, Virginia 24061, USA.
Magn Reson Chem. 2013 Sep;51(9):574-9. doi: 10.1002/mrc.3976. Epub 2013 Jun 10.
Antiproliferative bioassay-guided fractionation of the ethanol extract of the stems of Anisocycla grandidieri led to the isolation of the known alkaloids stebisimine (1), (+)-1,2-dehydrotelobine (2), (+)-2'-norcocsuline (3) and puetogaline B (4). Herein, we report the full NMR assignments of all compounds and the X-ray crystallography of single crystals of compounds 1 and 3. Compounds 2 and 3 showed moderate antiproliferative activity against the A2780 human ovarian cancer cell line with IC50 values of 4.1 ± 0.3 and 2.7 ± 0.3 μM, respectively, and they also displayed selective activity toward the H460 (large cell lung cancer), MCF-7 (breast ductal carcinoma), and UACC-257 (melanoma) cell lines.
对大花异环藤茎乙醇提取物进行抗增殖生物测定导向的分馏，得到了已知生物碱斯替比西明（1）、（+）-1,2-脱氢特洛宾（2）、（+）-2'-去甲考苏林（3）和普托加林B（4）。在此，我们报道了所有化合物的完整核磁共振归属以及化合物1和3单晶的X射线晶体学。化合物2和3对A2780人卵巢癌细胞系显示出中等抗增殖活性，IC50值分别为4.1±0.3和2.7±0.3μM，并且它们对H460（大细胞肺癌）、MCF-7（乳腺导管癌）和UACC-257（黑色素瘤）细胞系也表现出选择性活性。
