Ashrafi Seyed J, Rastegar Mahrokh F, Ashrafi Motahare, Yazdian Fatemeh, Pourrahim Reza, Suresh Anil K
Department of Life Science Engineering, University of Tehran, Tehran 14174, Iran.
J Nanosci Nanotechnol. 2013 Mar;13(3):2295-301. doi: 10.1166/jnn.2013.6791.
Naturally existing biological materials have been garning considerable attention as environmentally benign green-nanofactories for the fabrication of diverse nanomaterials, and with desired size and shape distributions. In the present investigation, we report the size and shape controllable biofabrication of silver nanocrystallites using the growth extract of the fungus, Rhizoctonia solani. Influence of various factors such as growth medium; radiation, in the form of sun light; and seeding duration on the production of silver nanoparticles using aqueous 1 mm silver nitrate solution under ambient conditions is presented. Our results demonstrate that these factors can significantly influence the production, size and shape transformation, and the rate of nanoparticles formation. Multiple characterization techniques involving UV-visible and Fourier transform infrared spectroscopy, X-ray diffraction, energy dispersive X-ray spectroscopy and transmission electron microscopy measurements confirmed the production, surface and structural characteristics, purity and crystalline nature of the biosynthesized silver nanoparticles. Our biogenic synthesis process provides a simple, ecologically friendly, cost-effective synthesis route, and most importantly the ability to have control over the size and shape distributions that lends itself for various biomedical and opto-electronic applications.
天然存在的生物材料作为用于制造各种具有所需尺寸和形状分布的纳米材料的环境友好型绿色纳米工厂，已引起了广泛关注。在本研究中，我们报告了使用真菌立枯丝核菌的生长提取物对银纳米晶体进行尺寸和形状可控的生物制造。介绍了各种因素，如生长培养基；以太阳光形式存在的辐射；以及在环境条件下使用1 mM硝酸银水溶液时接种持续时间对银纳米颗粒生产的影响。我们的结果表明，这些因素可显著影响银纳米颗粒的产量、尺寸和形状转变以及形成速率。涉及紫外可见光谱和傅里叶变换红外光谱、X射线衍射、能量色散X射线光谱和透射电子显微镜测量的多种表征技术证实了生物合成银纳米颗粒的产量、表面和结构特征、纯度及晶体性质。我们的生物合成过程提供了一种简单、生态友好、具有成本效益的合成路线，最重要的是能够控制尺寸和形状分布，这使其适用于各种生物医学和光电应用。
