曲霉 FC36AY1 源 AgONPs 的真菌制造及其强大的抗菌、抗氧化和抗血管生成活性。
Mycofabrication of AgONPs derived from Aspergillus terreus FC36AY1 and its potent antimicrobial, antioxidant, and anti-angiogenesis activities.
机构信息
Department of Biotechnology, Rathnavel Subramaniam College of Arts and Science, Coimbatore, Tamil Nadu, 641 402, India.
Department of Microbial Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu, 641 046, India.
出版信息
Mol Biol Rep. 2021 Dec;48(12):7933-7946. doi: 10.1007/s11033-021-06824-w. Epub 2021 Oct 16.
BACKGROUND
There is an emergency need for the natural therapeutic agents to treat arious life threatening diseases such as cardio- vascular disease, Rheumatoid arthritis and cancer. Among these diseases, cancer is found to be the second life threatening disease; in this view the present study focused to synthesize the silver oxide nanoparticles (AgONPs) from endophytic fungus.
METHODS
The endophytic fungus was isolated from a medicinal tree Aegle marmelos (Vilva tree) and the potential strain was screened through antagonistic activity. The endophytic fungus was identified through microscopic (Lactophenol cotton blue staining and spore morphology in culture media) and Internal Transcribed Spacer (ITS) 1, ITS 4 and 18S rRNA amplification. The endophyte was cultured for the synthesis of AgONPs and the synthesized NPs were characterized through UV- Vis, FT- IR, EDX, XRD and SEM. The synthesized AgONPs were determined for antimicrobial, antioxidant and anti- angiogenic activity.
RESULTS
About 35 pigmented endophytic fungi were isolated, screened for antagonistic activity against 12 pathogens and antioxidant activity through DPPH radical scavenging assay; among the isolates, FC36AY1 explored the highest activity and the strain FC36AY1 was identified as Aspergillus terreus. The AgONPs were synthesized from the strain FC36AY1 and characterized for its confirmation, functional groups, nanostructures with unit cell dimensions, size and shape, presence of elements through UV-Vis spectrophotometry, FT-IR, XRD, SEM with EDX analysis. The myco-generated AgONPs manifested their antimicrobial and antioxidant properties with maximum activity at minimum concentration. Moreover, the inhibition of angiogenesis by the AgONPs in Hen's Egg Test on the Chorio-Allantoic Membrane analysis were tested on the eggs of Chittagong breed evinced at significant bioactivity least concentration at 0.1 µg/mL.
CONCLUSIONS
Thus, the results of this study revealed that the fungal mediated AgONPs can be exploited as potential in biomedical applications.
背景
目前急需天然治疗剂来治疗各种危及生命的疾病，如心血管疾病、类风湿性关节炎和癌症。在这些疾病中，癌症被认为是第二大致命疾病；因此，本研究专注于从内生真菌中合成氧化银纳米粒子（AgONPs）。
方法
从药用树 Aegle marmelos（Vilva 树）中分离出内生真菌，并通过拮抗活性筛选出潜在菌株。通过显微镜（乳酚棉蓝染色和培养介质中的孢子形态）和内部转录间隔区（ITS）1、ITS 4 和 18S rRNA 扩增对内生真菌进行鉴定。内生真菌用于合成 AgONPs，并用紫外-可见分光光度计、傅里叶变换红外光谱仪（FT-IR）、能谱仪（EDX）、X 射线衍射仪（XRD）和扫描电子显微镜（SEM）对合成的 NPs 进行表征。合成的 AgONPs 用于抗菌、抗氧化和抗血管生成活性的测定。
结果
从 35 种有色内生真菌中分离出约 35 种内生真菌，对 12 种病原体进行拮抗活性筛选，并通过 DPPH 自由基清除试验进行抗氧化活性筛选；在这些分离物中，FC36AY1 表现出最高的活性，该菌株被鉴定为 Aspergillus terreus。从菌株 FC36AY1 中合成了 AgONPs，并通过紫外-可见分光光度计、傅里叶变换红外光谱仪、X 射线衍射仪、扫描电子显微镜与能谱分析对其进行了确认、功能基团、纳米结构与晶胞尺寸、大小和形状、元素的存在。从真菌中产生的 AgONPs 表现出其抗菌和抗氧化特性，在最低浓度下具有最大活性。此外，在鸡胚绒毛尿囊膜试验中对孟加拉鸡种鸡蛋进行了 AgONPs 抑制血管生成的测试，结果表明在最低浓度 0.1μg/mL 时具有显著的生物活性。
结论
因此，本研究结果表明，真菌介导的 AgONPs 可作为生物医学应用的潜在资源加以利用。
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