光学检测表观遗传标记：单个 5-羟甲基胞嘧啶碱基的灵敏定量和直接成像。

Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases.

机构信息

Raymond and Beverly Sackler Faculty of Exact Sciences, School of Chemistry, Tel Aviv University, Tel Aviv, Israel.

出版信息

Chem Commun (Camb). 2013 Oct 7;49(77):8599-601. doi: 10.1039/c3cc42543f.

Abstract

Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.

摘要

在此，我们报告了通过荧光报告分子对基因组 DNA 分子上的表观遗传修饰 5-羟甲基胞嘧啶进行特异性标记。酶促葡萄糖化后进行点击化学反应，可实现单个分子检测以及基因组 DNA 中 5hmC 的全局定量。