Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia.
J Virol. 2013 Aug;87(16):9384-90. doi: 10.1128/JVI.01101-13. Epub 2013 Jun 12.
NS1' is a C-terminally extended form of the NS1 protein produced only by encephalitic flaviviruses from the Japanese encephalitis virus serogroup. Here we show that West Nile virus (WNV) NS1' and NS1 localize to the same cellular compartments when expressed from plasmid DNAs and also colocalize to viral RNA replication sites in infected cells. Using complementation analysis with NS1-deleted WNV cDNA, we demonstrated that NS1' is able to substitute for the crucial function of NS1 in virus replication.
NS1' 是 NS1 蛋白的 C 端延伸形式，仅由日本脑炎病毒血清群的脑炎病毒产生。在这里，我们表明，西尼罗河病毒（WNV）的 NS1' 和 NS1 从质粒 DNA 表达时定位于相同的细胞区室，并且在感染细胞中也定位于病毒 RNA 复制位点。通过用 NS1 缺失的 WNV cDNA 进行互补分析，我们证明 NS1' 能够替代 NS1 在病毒复制中的关键功能。
