Department of Veterinary Pathobiology, Center for Veterinary Health Science, Oklahoma State University, Stillwater, OK 74078, USA.
Parasitol Res. 2013 Aug;112 Suppl 1:11-20. doi: 10.1007/s00436-013-3277-7.
Infection of Cytauxzoon felis in domestic cats produces a severe disease characterised by fever, lethargy, inappetence, anorexia, depression, dehydration, icterus and often death. Transmission of C. felis to cats is dependent on being fed upon by infected Amblyomma americanum (lone star ticks). The purpose of the present study was to determine if application of a 10 % imidacloprid/4.5 % flumethrin collar (Seresto®, Bayer) on cats prevents transmission of C. felis by repelling ticks. Twenty cats were randomised to either a treated (n = 10) or non-treated control group (n = 10) based on their susceptibility to ticks. Cats of high, medium and low tick susceptibility were represented in both groups. Treated cats were fitted with 10 % imidacloprid/4.5 % flumethrin collars on study day 0 and both groups were then infested with C. felis-infected A. americanum on study day 30. Tick thumb counts were performed at 24 and 48 hours post infestation. Transmission of C. felis was determined by examining blood of cats by DNA extraction followed by PCR amplification with piroplasm-specific primers. Ticks did not attach to any of the 10 % imidacloprid/4.5 % flumethrin- treated cats. However, ticks attached and fed on all the non-treated control cats. The geometric mean number of ticks attached to the non-treated control cats at 24 and 48 hours was 15.3 and 14.2, respectively. Cytauxzoon felis was transmitted to 9 of 10 (90 %) non-treated control cats; C. felis was not transmitted to any of the treated cats. Transmission of C. felis to the non-treated cats was first detected between 8 and 16 days post infestation. Our results indicate that application of the 10 % imidacloprid/4.5 % flumethrin collar to cats prevented ticks from attaching, feeding and transmitting C. felis.
猫感染 Cytauxzoon felis 会导致一种严重的疾病，其特征为发热、昏睡、食欲不振、厌食、抑郁、脱水、黄疸，且常常导致死亡。C. felis 向猫的传播取决于受感染的 Amblyomma americanum（孤星蜱）对其的叮咬。本研究的目的是确定在猫身上使用 10%吡虫啉/4.5%氟氯氰菊酯项圈（Seresto®，拜耳）是否可以通过驱避蜱来防止 C. felis 的传播。根据对蜱的易感性，将 20 只猫随机分为治疗组（n=10）或非治疗对照组（n=10）。两组均包括高、中、低易感性的猫。在研究日 0 时，治疗组的猫佩戴 10%吡虫啉/4.5%氟氯氰菊酯项圈，然后在研究日 30 时两组均用感染 C. felis 的 A. americanum 进行感染。在感染后 24 小时和 48 小时进行蜱拇指计数。通过提取血液中的 DNA 并使用小梨形虫特异性引物进行 PCR 扩增来确定 C. felis 的传播情况。蜱没有附着在任何 10%吡虫啉/4.5%氟氯氰菊酯处理的猫身上。然而，所有非治疗对照组的猫都有蜱附着并吸食。非治疗对照组的猫在 24 小时和 48 小时时附着的蜱的几何平均数量分别为 15.3 和 14.2。9/10（90%）只非治疗对照组的猫传播了 C. felis；没有任何治疗组的猫传播 C. felis。非治疗对照组的猫最早在感染后 8 至 16 天检测到 C. felis 的传播。我们的结果表明，将 10%吡虫啉/4.5%氟氯氰菊酯项圈应用于猫身上可防止蜱附着、吸食和传播 C. felis。
